GABPA Antibody (5C8) - Azide and BSA Free

Western Blot: GABPA Antibody (5C8) [H00002551-M07] - Analysis of GABPA expression in Hela S3 NE (Cat # L013V3).

Immunocytochemistry/Immunofluorescence: GABPA Antibody (5C8) [H00002551-M07] - Analysis of monoclonal antibody to GABPA on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: GABPA Antibody (5C8) [H00002551-M07] - Analysis of monoclonal antibody to GABPA on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml

ELISA: GABPA Antibody (5C8) [H00002551-M07] - Detection limit for recombinant GST tagged GABPA is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 5C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GABPA Antibody (5C8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GABPA (NP_002031, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTKREAEELIEIEIDGTEKAECTEESIVEQTYAPAECVSQAIDINEPIGNLKKLLEPRLQCSLDAHEICLQDIQLDPERSLFDQGVKTDGTVQLSVQVIS
• Specificity: GABPA (5C8)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GABPA
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GABPA Antibody (5C8) - Azide and BSA Free

• E4TF1-60
• E4TF1ATranscription factor E4TF1-60
• GA binding protein transcription factor, alpha subunit 60kDa
• GA-binding protein alpha chain
• GA-binding protein transcription factor, alpha subunit (60kD)
• GABP subunit alpha
• human nuclear respiratory factor-2 subunit alpha, 10
• NFT2
• NRF2
• NRF2A
• nuclear respiratory factor 2 alpha subunit
• Nuclear respiratory factor 2 subunit alpha

Background

This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GABPA
• Entrez:
  • 2551(Human)
• OMIM:
  • 600609(Human)
• Uniprot:
  • NP_002031(Human)