GABARAP Antibody (JM30-30)

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Western Blot: GABARAP Antibody (JM30-30) [NBP2-66854] - Analysis of GABARAP on mouse kidney (1) and rat liver (2) tissue lysate using anti-GABARAP antibody at 1/1,000 dilution.
Immunohistochemistry-Paraffin: GABARAP Antibody (JM30-30) [NBP2-66854] - Analysis of paraffin-embedded human placenta tissue using anti-GABARAP antibody. Counter stained with hematoxylin.
Flow Cytometry: GABARAP Antibody (JM30-30) [NBP2-66854] - Analysis of Hela cells with GABARAP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Immunohistochemistry-Paraffin: GABARAP Antibody (JM30-30) [NBP2-66854] - Analysis of paraffin-embedded human kidney tissue using anti-GABARAP antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: GABARAP Antibody (JM30-30) [NBP2-66854] - Analysis of paraffin-embedded human pancreas tissue using anti-GABARAP antibody. Counter stained with hematoxylin.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-66854
    • Availability
      Product Discontinued

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GABARAP Antibody (JM30-30) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within Human GABARAP aa 10-59 / 117. (SwissProt: O95166 Human; SwissProt: Q8R3R8 Mouse; SwissProt: P60517 Rat)
Localization
Endomembrane system. Cytoplasm, cytoskeleton.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
GABARAP
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for GABARAP Antibody (JM30-30)

  • Apg8p1
  • ATG8A
  • FLC3B
  • FLJ25768
  • GABA(A) receptor-associated proteinMGC120154
  • GABARAP
  • gamma-aminobutyric acid receptor-associated protein
  • MM46
  • MM46MGC120155

Background

FUNCTION: May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. SUBUNIT: Interacts with GABRG2, TUBA1, ULK1 and NSF. Interacts with beta-tubulin and GPHN. SUBCELLULAR LOCATION: Intracytoplasmic membrane. Cytoskeleton. Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules. SIMILARITY: Belongs to the MAP1 LC3 family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Research Areas for GABARAP Antibody (NBP2-66854)

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Blogs on GABARAP.


  Read full blog post.

Breakdown: Interpreting LC3 Antibody WB Results
In rodents, MAP1LC3 (Microtubule-associated protein 1 light chain 3) is expressed in the renal visceral epithelial cells, or podocytes. LC3 antibody analysis has shown the protein accumulates in its membrane-bound form, LC3II, following conversio...  Read full blog post.

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Bioinformatics

Gene Symbol GABARAP