GABA-A R beta 3 Antibody

Immunohistochemistry-Paraffin: GABA-A R beta 3 Antibody [NBP1-06988] - (2.5ug/ml) staining of Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GABA-A R beta 3 Antibody Summary

• Immunogen: Peptide with sequence C-EKTAKAKNDRSK corresponding to internal region according to NP_068712.1.
• Epitope: internal region
• Specificity: This antibody is expected to recognize both reported isoforms (NP_000805.1, NP_068712.1).
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GABRB3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:8000
• Application Notes: WB: Preliminary experiments in human brain (cerebral cortex, frontal cortex and cerebellum) lysates gave no specific signal but low background. IHC-P: Human cortex shows patchy staining near or at the neuronal cell membrane.
• Control:

  GABA-A R beta 3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GABA-A R beta 3 Antibody

• ECA5
• GABA A R beta 3
• GABA(A) receptor beta-3 subunit
• GABA(A) receptor subunit beta-3
• GABAA receptor beta-3 subunit
• GABA-alpha receptor beta-2 subunit
• GABAAR beta 3
• GABAARb3
• GABRB3
• gamma-aminobutyric acid (GABA) A receptor, beta 3
• gamma-aminobutyric acid receptor subunit beta-3
• MGC9051

Background

The GABRB3 gene encodes a member of the ligand-gated ionic channel family. The encoded protein, GABA A Receptor beta 3, is one of at least 13 distinct subunits of a multisubunit chloride channel that serves as the receptor for gamma-aminobutyric acid, the major inhibitory transmitter of the nervous system. GABRB3 is located on the long arm of chromosome 15 in a cluster with two genes encoding related subunits of the family. Mutations in the GABA A Receptor beta 3 gene may be associated with the pathogenesis of Angelman syndrome, Prader-Willi syndrome, and autism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GABA-A R beta 3 Antibody (NBP1-06988)(1)

Publication using NBP1-06988

• Tanaka M, Olsen RW, Medina MT et al. Hyperglycosylation and reduced GABA currents of mutated GABRB3 polypeptide in remitting childhood absence epilepsy. Am J Hum Genet 2008-06-01 [PMID: 18514161]

Bioinformatics

• Gene Symbol: GABRB3
• Entrez:
  • 2562(Human)
  • 14402(Mouse)
  • 24922(Rat)
• Uniprot:
  • NP_000805.1(Human)