GABA-A R beta 1 Antibody - Azide Free

Western Blot: GABA-A R beta 1 Antibody [NB300-197] - Mouse whole brain lysates showing specificimmunolabeling of the ~55 kDa beta1-subunit of the GABAA-R.

Western Blot: GABA-A R beta 1 Antibody [NB300-197] - Rat cerebellar (Cb) and hippocampal (Hipp) lysates showing specific immunolabeling of the ~55k beta 1-subunit of the GABA-A R beta 1 Antibody.

• Reactivity: Mu, Rt, Hu, Bv, Ca, Ch, Pm, Xp, Ze
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

GABA-A R beta 1 Antibody - Azide Free Summary

• Immunogen: Fusion protein from the cytoplasmic loop of the beta 1 subunit. Accession # P15431
• Localization: Integral membrane protein
• Specificity: Specific for the ~55 kDa beta 1-subunit of the GABA A receptor in Western blots.
• Predicted Species: Human (100%), Chicken (100%), Canine (100%), Xenopus (100%), Primate (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GABRB1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:1000
• Application Notes: This antibody is useful in Immunohistochemistry (PMID: 21628464). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 21628464)
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate, Xenopus, and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for GABA-A R beta 1 Antibody - Azide Free

• GABA A R beta 1
• GABA(A) receptor subunit beta-1
• GABAAR beta 1
• GABAARb1
• GABRB1
• gamma-aminobutyric acid (GABA) A receptor, beta 1
• gamma-aminobutyric acid receptor subunit beta-1

Background

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GABA-A R beta 1 Antibody (NB300-197)(1)

Publication using NB300-197

• Gutierrez DV, Mark MD, Masseck O et al. Optogenetic control of motor coordination by Gi/o protein-coupled vertebrate rhodopsin in cerebellar Purkinje cells. J Biol Chem. 2011-07-01 [PMID: 21628464] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: GABRB1
• Entrez:
  • 2560(Human)
  • 14400(Mouse)
  • 25450(Rat)
• Uniprot:
  • P15431(Rat)