G9a/EHMT2 Antibody (A8620A) [Unconjugated]

G9a is not required for the transcriptional repression of selected early embryonic genes during retinoic acid (RA)-induced differentiation. The indicated mESC lines were untreated (0) or treated with 1 μM RA for 2, 4 or 8 days. (A) Immunoblots were used to determine G9a, Oct3/4 and GAPDH (loading control) protein levels in whole cell extracts. (B) Graph shows the messenger (mRNA) levels for the indicated genes using RT-qPCR analysis with Ubiquitin C (UBC) as the normalization control. All mRNA levels are normalized to the TT2 0 RA sample. Results shown are mean ± SD for three PCR reactions performed on the same cDNA sample and are representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25478012), licensed under a CC-BY license. Not internally tested by R&D Systems.

G9a expression is positively correlated with cell proliferation and poor prognosis in HNSCC patients. (A) Correlation between Ki-67 and G9a expression. The expression of two proteins were analyzed by IHC staining of tissue microarray (TMA) containing 46 normal and 108 tumor sections from HNSCC patients. The expression level of both Ki-67 and G9a was measured by relative staining intensity multiplied by percentage. Scale bar, 50 μm. (*, p < 0.05; ***, p < 0.001). (B) Ki-67 and G9a IHC staining of serial section of tumors. Low or high Ki-67 and G9a expression were defined by a threshold of 80 and 143, which is the average of relative staining intensity multiplied by percentage of Ki-67 and G9a. Scale bar, 50 μm. (***, p < 0.001). (C) Kaplan-Meier plot of overall survival of 77 HNSCC patients from a National Taiwan University Hospital cohort stratified by G9a expression level. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25027955), licensed under a CC-BY license. Not internally tested by R&D Systems.

G9a expression is positively correlated with cell proliferation and poor prognosis in HNSCC patients. (A) Correlation between Ki-67 and G9a expression. The expression of two proteins were analyzed by IHC staining of tissue microarray (TMA) containing 46 normal and 108 tumor sections from HNSCC patients. The expression level of both Ki-67 and G9a was measured by relative staining intensity multiplied by percentage. Scale bar, 50 μm. (*, p < 0.05; ***, p < 0.001). (B) Ki-67 and G9a IHC staining of serial section of tumors. Low or high Ki-67 and G9a expression were defined by a threshold of 80 and 143, which is the average of relative staining intensity multiplied by percentage of Ki-67 and G9a. Scale bar, 50 μm. (***, p < 0.001). (C) Kaplan-Meier plot of overall survival of 77 HNSCC patients from a National Taiwan University Hospital cohort stratified by G9a expression level. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25027955), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP, ELISA
• Clone: A8620A
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Suitable Sample Type: 23005

G9a/EHMT2 Antibody (A8620A) [Unconjugated] Summary

• Immunogen: Recombinant mouse G9a/EHMT2
  aa 15-114
• Specificity: This antibody specifically recognizes mouse G9a and cross-reacts with human G9a. Not yet tested in other species.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: EHMT2
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Direct ELISA
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: This antibody is stable for greater than six months when held at -20° C in a manual defrost freezer or at -70°C. Upon thawing, the antibody can be stored at 2° - 8° C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
• Buffer: A liquid formulation in physiologic saline with 0.1% NaN₃.
• Preservative: 0.1% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for G9a/EHMT2 Antibody (A8620A) [Unconjugated]

• ankyrin repeat-containing protein
• BAT8
• C6orf30
• chromosome 6 open reading frame 30
• DKFZp686H08213
• EC 2.1.1.-
• EC 2.1.1.43
• EHMT2
• euchromatic histone-lysine N-methyltransferase 2HLA-B-associated transcript 8
• FLJ35547
• G9A histone methyltransferase
• G9a
• G9AEm:AF134726.3
• H3-K9-HMTase 3
• Histone H3-K9 methyltransferase 3
• histone-lysine N-methyltransferase, H3 lysine-9 specific 3
• HLA-B associated transcript 8
• KMT1CNG36/G9a
• Lysine N-methyltransferase 1C
• NG36
• Protein G9a

Background

Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic marker for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential for mouse embryogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for G9a/EHMT2 Antibody (PP-A8620A-00)(19)

Publications using PP-A8620A-00

• Huang, Z;Cui, W;Ratnayake, I;Gallik, KL;Cohen, L;Tawil, R;Pfeifer, GP; SMCHD1 maintains heterochromatin, genome compartments and epigenome landscape in human myoblasts Nature communications 2025-07-26 [PMID: 40715155] (Western Blot, Human)
• Huang, Z;Cui, W;Ratnayake, I;Tawil, R;Pfeifer, GP; SMCHD1 maintains heterochromatin and genome compartments in human myoblasts bioRxiv : the preprint server for biology 2024-07-10 [PMID: 39026812] (Western Blot, Human)
• Hsu, CM;Chang, KC;Chuang, TM;Chu, ML;Lin, PW;Liu, HS;Kao, SY;Liu, YC;Huang, CT;Wang, MH;Yeh, TJ;Gau, YC;Du, JS;Wang, HC;Cho, SF;Hsiao, CE;Tsai, Y;Hsiao, SY;Hung, LC;Yen, CH;Hsiao, HH; High G9a Expression in DLBCL and Its Inhibition by Niclosamide to Induce Autophagy as a Therapeutic Approach Cancers 2023-08-17 [PMID: 37627178] (IHC, Human)
• Able, AA;Richard, AJ;Stephens, JM; TNF? Effects on Adipocytes Are Influenced by the Presence of Lysine Methyltransferases, G9a (EHMT2) and GLP (EHMT1) Biology 2023-04-30 [PMID: 37237488] (Western Blot, Mouse)
• LT Yuan, WJ Lee, YC Yang, BR Chen, CY Yang, MW Chen, JQ Chen, M Hsiao, MH Chien, KT Hua Histone Methyltransferase G9a-Promoted Progression of Hepatocellular Carcinoma Is Targeted by Liver-Specific Hsa-miR-122 Cancers, 2021-05-14;13(10):. 2021-05-14 [PMID: 34069116] (IHC, Human)
• T Nakatsuka, K Tateishi, H Kato, H Fujiwara, K Yamamoto, Y Kudo, H Nakagawa, Y Tanaka, H Ijichi, T Ikenoue, T Ishizawa, K Hasegawa, M Tachibana, Y Shinkai, K Koike Inhibition of histone methyltransferase G9a attenuates liver cancer initiation by sensitizing DNA-damaged hepatocytes to p53-induced apoptosis Cell Death & Disease, 2021-01-19;12(1):99. 2021-01-19 [PMID: 33468997] (IHC, Western Blot, Mouse)
• Tian H, Billings T, Walker M et al. EWSR1 affects PRDM9-dependent histone 3 methylation and provides a link between recombination hotspots and the chromosome axis Mol Biol Cell 2020-11-11 [PMID: 33175657]
• Z Li, X Jiao, G Di Sante, A Ertel, MC Casimiro, M Wang, S Katiyar, X Ju, DV Klopfenste, A Tozeren, W Dampier, I Chepelev, A Jeltsch, RG Pestell Cyclin D1 integrates G9a-mediated histone methylation Oncogene, 2019-02-04;0(0):. 2019-02-04 [PMID: 30718920] (ChIP, ICC, Western Blot, Mouse)
• L Li, W Liu, H Wang, Q Yang, L Zhang, F Jin, Y Jin Mutual inhibition between HDAC9 and miR-17 regulates osteogenesis of human periodontal ligament stem cells in inflammatory conditions Cell Death Dis, 2018-05-01;9(5):480. 2018-05-01 [PMID: 29691366] (Western Blot, Human)
• RJ Chen, CT Shun, ML Yen, CH Chou, MC Lin Methyltransferase G9a promotes cervical cancer angiogenesis and decreases patient survival Oncotarget, 2017-07-07;8(37):62081-62098. 2017-07-07 [PMID: 28977928] (IHC, Human)
• Ozge Gursoy-Yuzugullu, Chelsea Carman, Rodolfo Bortolozo Serafim, Marios Myronakis, Valeria Valente, Brendan D. Price Epigenetic therapy with inhibitors of histone methylation suppresses DNA damage signaling and increases glioma cell radiosensitivity Oncotarget 2017-04-11 [PMID: 28445939]
• CW Liu, KT Hua, KC Li, HF Kao, RL Hong, JY Ko, M Hsiao, ML Kuo, CT Tan Histone methyltransferase G9a drives chemotherapy resistance by regulating the glutamate-cysteine ligase catalytic subunit in head and neck squamous cell carcinoma Mol. Cancer Ther, 2017-03-06;0(0):. 2017-03-06 [PMID: 28265008] (IHC-P, Human)
• Pei Han, Wei Li, Jin Yang, Ching Shang, Chiou-Hong Lin, Wei Cheng et al. Epigenetic response to environmental stress: Assembly of BRG1–G9a/GLP–DNMT3 repressive chromatin complex on Myh6 promoter in pathologically stressed hearts☆,☆☆ Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 2016-07-01 [PMID: 26952936]
• Ghislain Auclair, Julie Borgel, Lionel A. Sanz, Judith Vallet, Sylvain Guibert, Michael Dumas et al. EHMT2 directs DNA methylation for efficient gene silencing in mouse embryos Genome Research 2016-02-01 [PMID: 26576615]
• Jan J Zylicz, Sabine Dietmann, Ufuk Günesdogan, Jamie A Hackett, Delphine Cougot, Caroline Lee et al. Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development eLife 2015-11-09 [PMID: 26551560] (Immunohistochemistry, Transgenic Mouse)
• Li , Kai-Chun, Hua , Kuo-Tai, Lin , Yi-Shen, Su , Chia-Yi, Ko , Jenq-Yuh, Hsiao , Michael, Kuo , Min-Lian, Tan , Ching-Ti Inhibition of G9a induces DUSP4-dependent autophagic cell death in head and neck squamous cell carcinoma. Mol Cancer, 2014-07-15;13(0):172. 2014-07-15 [PMID: 25027955] (IHC, Human)
• Lehnertz B, Pabst C, Su L, Miller M, Liu F, Yi L, Zhang R, Krosl J, Yung E, Kirschner J, Rosten P, Underhill T, Jin J, Hebert J, Sauvageau G, Humphries R, Rossi F The methyltransferase G9a regulates HoxA9-dependent transcription in AML. Genes Dev, 2014-02-15;28(4):317-27. 2014-02-15 [PMID: 24532712] (Western Blot, Human)
• Chen MW, Hua KT, Kao HJ, Chi CC, Wei LH, Johansson G, Shiah SG, Chen PS, Jeng YM, Cheng TY, Lai TC, Chang JS, Jan YH, Chien MH, Yang CJ, Huang MS, Hsiao M, Kuo ML H3K9 histone methyltransferase G9a promotes lung cancer invasion and metastasis by silencing the cell adhesion molecule Ep-CAM. Cancer Res., 2010-10-12;70(20):7830-40. 2010-10-12 [PMID: 20940408] (IHC-P, Human)
• Dong K, Maksakova I, Mohn F, Leung D, Appanah R, Lee S, Yang H, Lam L, Mager D, Schubeler D, Tachibana M, Shinkai Y, Lorincz M DNA methylation in ES cells requires the lysine methyltransferase G9a but not its catalytic activity. EMBO J, 2008-09-25;27(20):2691-701. 2008-09-25 [PMID: 18818693] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: EHMT2