G protein alpha inhibitor 1 Antibody - BSA Free

Western Blot: G protein alpha inhibitor 1 Antibody - BSA Free [NBP3-46210] - Human brain tissue were subjected to SDS PAGE followed by western blot with (G protein alpha inhibitor 1 antibody) at dilution of 1:1600

Immunohistochemistry: G protein alpha inhibitor 1 Antibody - BSA Free [NBP3-46210] - Immunohistochemistry of paraffin-embedded human gliomas using (G protein alpha inhibitor 1 antibody) at dilution of 1:100

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, NULL
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

G protein alpha inhibitor 1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit G protein alpha inhibitor 1 Antibody - BSA Free (NBP3-46210) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: guanine nucleotide binding protein(G protein), alpha inhibiting activity polypeptide 1. (Uniprot# P63096)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GNAI1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200
  • Immunohistochemistry 1:20-1:200
  • Serum FC
  • Western Blot 1:500-1:2000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for G protein alpha inhibitor 1 Antibody - BSA Free

• Adenylate cyclase-inhibiting G alpha protein
• Gi
• Gi1 protein alpha subunit
• guanine nucleotide binding protein (G protein), alpha inhibiting activitypolypeptide 1
• guanine nucleotide-binding protein G(i) subunit alpha-1

Background

Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs (MIM 139320) and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein abundant in brain (GNAO1; MIM 139311); and transducin-1 (GNAT1; MIM 139330) and transducin-2 (GNAT2; MIM 139340), proteins involved in phototransduction in retinal rods and cones, respectively (Sullivan et al., 1986 [PubMed 3092218]; Bray et al., 1987 [PubMed 3110783]). Suki et al. (1987) [PubMed 2440724] concluded that the human genome contains at least 3 nonallelic genes for alpha-i-type subunits of G protein; see, e.g, GNAI2 (MIM 139360), GNAI3 (MIM 139370), and GNAIH (MIM 139180).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GNAI1