G protein alpha inhibitor 1/2 Antibody

Immunofluorescence: G protein alpha inhibitor 1/2 Antibody [NB120-3522] - Analysis of G Protein alpha Inhibitor 1 and 2 was performed using 70% confluent log phase SH-SY5Y cells.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Fi, Gp, Ha, Xp
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

G protein alpha inhibitor 1/2 Antibody Summary

• Description: Novus Biologicals Rabbit G protein alpha inhibitor 1/2 Antibody (NB120-3522) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to residues K(346) N N L K D C G L F(355) of human G Protein alpha Inhibitor 1/2.
• Localization: Plasma membrane
• Specificity: NB120-3522 detects G protein alpha inhibitor 1 (Gene ID: 2770, UniProt: P63096), and G protein alpha inhibitor 2 (Gene ID: 2771, UniProt: P04899) from human, rat, and mouse tissues and cells.
• Predicted Species: Bovine (100%), Guinea Pig (100%), Fish (100%), Canine (100%), Hamster (100%), Chicken (100%), Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GNAI1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: WB: Detects an approx. 38 kDa protein representing G protein alpha inhibitor 1/2 from HeLa cell lysate.

Reactivity Notes

Expected cross-reactivity with Hydra, Octopus, Spiny Caninefish, Great Pond Snail, Starfish, and American Lobster (100%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for G protein alpha inhibitor 1/2 Antibody

• GIP
• GNAI1
• GNAI1/2
• GNAI2
• GNAI2B
• H_LUCA15.1

Background

The G protein family of signal transducers includes the alpha, beta, gamma subunits and the effector which are most clearly distinguished by their different alpha chains. The family also includes Gs and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein of unknown function that is abundantly expressed in brain; and transducin 1 and 2, proteins involved in retinal phototransduction. The G proteins possess GTPase activity and are components of a complex membrane signaling system that includes membrane-bound receptors. The system exists to modulate extracellular signals as they are transmitted into the cell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GNAI1