G-CSF Antibody (5008) [Phycoerythrin]

Human CD14+ PBMC treated with Recombinant Human IL-4 (20 ng/ml; 204-IL), Recombinant Human GM-CSF (50 ng/ml; 215-GM), LPS (20 ng/ml), Recombinant Human TNF-alpha (20 ng/ml; 210-TA), and Recombinant Human IL-beta (20 ng/ml; 201-LB) for 6 days to differentiate into Dendritic Cells were stained with Mouse Anti-Human G-CSF PE-conjugated Monoclonal Antibody (Catalog # IC2141P, filled histogram) or isotype control antibody (IC002P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.

Human macrophages treated with LPS, Recombinant Human TNF-a (Catalog # 210-TA), and Recombinant Human IL-beta (Catalog # 201-LB) were stained with Mouse Anti-Human G-CSF PE-conjugated Monoclonal Antibody (Catalog # IC2141P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 5008
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

G-CSF Antibody (5008) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human G-CSF
  Thr31-Pro204
  Accession # NP_757373
• Specificity: Detects human G-CSF in flow cytometry.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSF3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8degrees C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for G-CSF Antibody (5008) [Phycoerythrin]

• C17orf33
• chromosome 17 open reading frame 33
• colony stimulating factor 3 (granulocyte)
• CSF3
• CSF3OS
• Filgrastim
• GCSF
• G-CSF
• GCSFlenograstim
• granulocyte colony-stimulating factor
• Lenograstim
• MGC45931
• Pluripoietin

Background

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma . Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1, or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity. In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the G o period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for G-CSF Antibody (IC2141P)(1)

Publication using IC2141P

• Uchida K, Nakata K, Trapnell BC, Terakawa T, Hamano E, Mikami A, Matsushita I, Seymour JF, Oh-Eda M, Ishige I, Eishi Y, Kitamura T, Yamada Y, Hanaoka K, Keicho N High-affinity autoantibodies specifically eliminate granulocyte-macrophage colony-stimulating factor activity in the lungs of patients with idiopathic pulmonary alveolar proteinosis. Blood, 2003-09-25;103(3):1089-98. 2003-09-25 [PMID: 14512323] (IHC-P, Human)

Bioinformatics

• Gene Symbol: CSF3
• Uniprot:
  • NP_757373()