FXYD5/Dysadherin Antibody

Images

 
Western Blot: FXYD5/Dysadherin Antibody [NB100-1003] - (0.5ug/ml) of Human Spleen lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

FXYD5/Dysadherin Antibody Summary

Immunogen
Peptide with sequence GKCRQLSRLCRNHCR corresponding to C-Terminus according to NP_054883.3, NP_659003.1, NP_001158077.1.
Specificity
NP_054883.3, NP_659003.1and NP_001158077.1 are variants that represent the same protein.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FXYD5
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Preliminary experiments gave an approx. 38 kDa band in human spleen lysate and an approx. 26 kDa band in human lung lysate after 1 ug/ml antibody staining. The bands were successfully blocked by incubation with the immunizing peptide. Please note that according to the literature this protein is O-glycosylatedscientific literature (PMID: 12672699), which may explain the different sizes of bands we observe given the calculated size of 19.5 kDa band according to NP_054883 and NP_659003.
Positive Control
FXYD5/Dysadherin Lysate (NBL1-10880)
Publications
Read Publications using NB100-1003.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FXYD5/Dysadherin Antibody

  • DYSAD
  • Dysadherin
  • EF-8
  • FXYD domain containing ion transport regulator 5
  • FXYD domain-containing ion transport regulator 5
  • FXYD5
  • IWU1
  • KCT1
  • keratinocytes associated transmembrane protein 1
  • OIT2
  • PRO6241
  • RIC

Background

This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FXYD5/Dysadherin Antibody (NB100-1003)(2)

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FXYD5
Entrez
Uniprot