Furin Overexpression Lysate (Native)


Western Blot: Furin Overexpression Lysate (Adult Normal) [NBL1-10860] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Furin.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

Furin Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC204279 and based on accession number NM_002569. The protein contains a C-terminal DDK tag.
Homo sapiens furin (paired basic amino acid cleaving enzyme) (FURIN), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
86.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for Furin

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Furin Overexpression Lysate (Native)

  • Dibasic-processing enzyme
  • EC 3.4.21
  • EC
  • FUR
  • FURdibasic processing enzyme
  • furin (paired basic amino acid cleaving enzyme)
  • furin, membrane associated receptor protein
  • PACE
  • PACEFES upstream region
  • paired basic amino acid cleaving enzyme (furin, membrane associated receptorprotein)
  • Paired basic amino acid residue-cleaving enzyme
  • PC
  • PCSK3
  • PCSK3furin
  • proprotein convertase subtilisin/kexin type 3
  • SPC1


The protein encoded by this gene belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a calcium-dependent serine endoprotease that can efficiently cleave precursor proteins at their paired basic amino acid processing sites. Some of its substrates are: proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is also thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140. This gene is thought to play a role in tumor progression. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Furin Lysate (NBL1-10860) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Contact Information

Product PDFs


Gene Symbol FURIN

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