Cytokeratin 17 Antibody

Images

 
Western Blot: Cytokeratin 17 Antibody [NBP2-38536] - Analysis in human cell lines HeLa and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 17 Antibody [NBP2-38536] - MYP3 rat urothelial cell line with CK17 (green) and DAPI (blue). Primary antibody at 1:200. Image from verified customer review.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP2-38536] - Staining in human urinary bladder and endometrium tissues using anti-KRT17 antibody. Corresponding KRT17 RNA-seq data ...read more
Western Blot: Cytokeratin 17 Antibody [NBP2-38536] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: Cytokeratin 17 Antibody [NBP2-38536] - Staining of human cell line hTCEpi shows localization to intermediate filaments. Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 17 Antibody [NBP2-38536] - UROtsa (rat urothelial cell line). CK17 in green, DAPI in blue. Secondary antibody: Goat Polyclonal Anti-Rabbit at 1:500. Image from ...read more
Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP2-38536] - Staining of human endometrium shows low expression as expected.
Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP2-38536] - Staining of human urinary bladder shows high expression.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Cytokeratin 17 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SSRTSCRLSGGLGAGSCRLGSAGGL
Specificity
Specificity of human Cytokeratin 17 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (92%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KRT17
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Cytokeratin 17 Protein (NBP2-38536PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP2-38536 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Cytokeratin 17 Antibody

  • 39.1
  • CK-17
  • cytokeratin-17
  • K17
  • keratin 17
  • keratin, type I cytoskeletal 17
  • keratin-17
  • PC
  • PC2
  • PCHC1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytokeratin 17 Antibody (NBP2-38536) (0)

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Review for Cytokeratin 17 Antibody (NBP2-38536) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NBP2-38536:
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IF
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence Cytokeratin 17 NBP2-38536
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reviewed by:
Anonymous
IF Rat 05/18/2018
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Summary

ApplicationImmunofluorescence
Sample Testedbladder cell line
SpeciesRat
LotA115310

Comments

Comments1/200 dilution (would recommend higher in the future) with UROtsa rat urothelial cells. Secondary antibody of Goat Polyclonal Anti-Rabbit (Invitrogen) at 1/500 dilution.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin 17 Antibody (NBP2-38536) (0)

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Secondary Antibodies

 

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Diseases for Cytokeratin 17 Antibody (NBP2-38536)

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Recent Reviews

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Anonymous
05/18/2018
Application: IF
Species: Rat

Bioinformatics

Gene Symbol KRT17
Uniprot