Furin Antibodies
Furin is a calcium-dependent serine protease belonging to the subtilisin-like proprotein convertase (PC) family with the pre-pro form at a theoretical molecular weight of 87 kDa, typically running around 105 kDa on a Western Blot due post-translational modifications. Besides functioning in embryogenesis and the maturation of proprotein substrates, Furin processes the SARS-CoV-2 (COVID-19) Spike protein at the S1/S2 cleavage site, enabling binding of Spike RBD to ACE-2 for viral entry. From our extensive catalog, we offer antibodies to detect Furin including polyclonal antibodies, monoclonal antibodies, conjugated antibodies, and a recombinant rabbit monoclonal antibody. Validated research applications include Block/Neutralize, ELISA, ICC, Immunofluorescence, IHC, IHC-Paraffin, IP, CyTOF-ready, Flow Cytometry, and Western Blot. Our Furin antibodies can be used in a variety of model species including Human, Mouse, Rat, Primate, Canine, Equine, Porcine, Bovine and Hamster. Choose the anti-Furin antibody best suited for your research needs by viewing the technical details, images, references, reviews and related products. Each Furin antibody is fully covered by our Guarantee+.
Alternate Names for Furin Antibodies
anti-Furin antibody, anti-Dibasic-processing enzyme antibody, anti-EC 3.4.21 antibody, anti-EC 3.4.21.75 antibody, anti-FURdibasic processing enzyme antibody, anti-furin (paired basic amino acid cleaving enzyme) antibody, anti-furin, membrane associated receptor protein antibody, anti-PACEFES upstream region antibody, anti-paired basic amino acid cleaving enzyme (furin, membrane associated receptorprotein) antibody, anti-Paired basic amino acid residue-cleaving enzyme antibody, anti-PCSK3furin antibody, anti-proprotein convertase subtilisin/kexin type 3 antibody, anti-SPC1 antibody
37 Results for "furin" in Primary Antibodies
![Western Blot: Furin Antibody [NB100-1903] - Analysis was performed on membrane enriched extracts (30 ug lysate) of HeLa (Lane 1), HEK 293 (Lane 2), K-562 (Lane 3), SH-SY5Y (Lane 4) and 10uL conditioned media from HeLa cell line (Lane 5).](http://images.novusbio.com/fullsize/Furin-Antibody-Western-Blot-NB100-1903-img0008.jpg "Western Blot: Furin Antibody [NB100-1903] - Analysis was performed on membrane enriched extracts (30 ug lysate) of HeLa (Lane 1), HEK 293 (Lane 2), K-562 (Lane 3), SH-SY5Y (Lane 4) and 10uL conditioned media from HeLa cell line (Lane 5).")
![Immunocytochemistry/Immunofluorescence: Furin Antibody [NB100-1903] - Immunolocalization of endogenous furin in mouse 3T3 cells.](http://images.novusbio.com/fullsize/Furin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1903-img0005.jpg)
![Flow Cytometry: Furin Antibody [NB100-1903] - Flow cytometry analysis of Furin Convertase was done on HeLa cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton (R) X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Furin Convertase Rabbit Polyclonal Antibody or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor (R) 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10, 000 cells were acquired and analyzed for each sample using an Attune (R) Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.](http://images.novusbio.com/fullsize/Furin-Antibody-Flow-Cytometry-NB100-1903-img0007.jpg)
![Immunohistochemistry-Paraffin: Furin Antibody [NBP2-24687] - analysis of Furin in formalin-fixed, paraffin-embedded human kidney tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/Furin-Antibody-Immunohistochemistry-Paraffin-NBP2-24687-img0001.jpg "Immunohistochemistry-Paraffin: Furin Antibody [NBP2-24687] - analysis of Furin in formalin-fixed, paraffin-embedded human kidney tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Furin Antibody [NBP2-26104] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue) Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Furin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-26104-img0002.jpg "Immunocytochemistry/Immunofluorescence: Furin Antibody [NBP2-26104] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue) Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunocytochemistry/Immunofluorescence: Furin Antibody [NBP2-26104] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Furin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-26104-img0001.jpg)
![Immunohistochemistry-Paraffin: Furin Antibody (MM0298-4G31) [NBP2-12321] - 10% Buffer formalin fixed and parffine embedded human placetal tissue section (4um) is subjected IHC staining using NBP2-12321. Tissue section was pretreated in citric buffer (pH 6.0) with microwave for antige retrieval before IHC is applied.](http://images.novusbio.com/fullsize/Furin-Antibody-MM0298-4G31-Immunohistochemistry-Paraffin-NBP2-12321-img0001.jpg "Immunohistochemistry-Paraffin: Furin Antibody (MM0298-4G31) [NBP2-12321] - 10% Buffer formalin fixed and parffine embedded human placetal tissue section (4um) is subjected IHC staining using NBP2-12321. Tissue section was pretreated in citric buffer (pH 6.0) with microwave for antige retrieval before IHC is applied.")
![Immunohistochemistry: Furin Antibody (1C3) [NBP3-26563] - Image of Furin Antibody (1C3) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26563_rabbit-furin-mab-1c3-262202415253310.jpg "Immunohistochemistry: Furin Antibody (1C3) [NBP3-26563] - Image of Furin Antibody (1C3) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Furin Antibody (1C3) [NBP3-26563] - Positive Western Blot detected in: A549 whole cell lysate, Hela whole cell lysate, A375 whole cell lysate, HT-29 whole cell lysate, HepG2 whole cell lysate, U-87 whole cell lysate. All lanes: Furin Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 87 kDa Observed band size: 87, 72 kDa](http://images.novusbio.com/fullsize/nbp3-26563_rabbit-furin-mab-1c3-282202411485847.jpg)
![Western Blot: Furin Antibody (1P6N8) [NBP3-16535] - Western blot analysis of extracts of various cell lines, using Furin Rabbit mAb (NBP3-16535) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Furin-Antibody-1P6N8-Western-Blot-NBP3-16535-img0001.jpg "Western Blot: Furin Antibody (1P6N8) [NBP3-16535] - Western blot analysis of extracts of various cell lines, using Furin Rabbit mAb (NBP3-16535) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunoprecipitation: Furin Antibody (1P6N8) [NBP3-16535] - Immunoprecipitation analysis of 300ug extracts of A-549 cells using 3ug Furin antibody (NBP3-16535). Western blot was performed from the immunoprecipitate using Furin antibody (NBP3-16535) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Furin-Antibody-1P6N8-Immunoprecipitation-NBP3-16535-img0002.jpg)
![Western Blot: Furin Antibody [NBP3-07955] - Western blot analysis of extracts of various cell lines, using Rabbit Monoclonal Furin Antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blockin buffer: 3% nonfat dry milk TBST. Exposure time: 90s.](http://images.novusbio.com/fullsize/Furin-Antibody-Western-Blot-NBP3-07955-img0001.jpg "Western Blot: Furin Antibody [NBP3-07955] - Western blot analysis of extracts of various cell lines, using Rabbit Monoclonal Furin Antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blockin buffer: 3% nonfat dry milk TBST. Exposure time: 90s.")
