Fumarase Antibody (2E10)

Product Discontinued

Fumarase Antibody (2E10) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: FH (AAH03108, 33 a.a. - 510 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VPSFWPPNAARMASQNSFRIEYDTFGELKVPNDKYYGAQTVRSTMNFKIGGVTERMPTPVIKAFGILKRAAAEVNQDYGLDPKIANAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNVNEVISNRAIEMLGGELGSKIPVHPNDHVNKSQSSNDTFPTAMHIAAAIEVHEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK
• Specificity: FH - fumarate hydratase (2E10)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FH
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Fumarase Antibody (2E10)

• EC 4.2.1.2
• fumarase
• fumarate hydratase
• fumarate hydratase, mitochondrial
• HLRCC
• LRCC
• MCL
• MCUL1

Background

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fumarase Antibody (H00002271-M01)(3)

Publications using H00002271-M01

• Kratochvilova Hana, Hejzlarova Katerina, Vrbacky Marek et al. Mitochondrial membrane assembly of TMEM70 protein. Mitochondrion. 2014-02-25 [PMID: 24576557] (Human)
• Kovarova N, Mracek T, Nuskova H et al. High Molecular Weight Forms of Mammalian Respiratory Chain Complex II. PLoS One. 2013-08-13 [PMID: 23967256]
• Xie H, Valera VA, Merino MJ et al. LDH-A inhibition, a therapeutic strategy for treatment of hereditary leiomyomatosis renal cell cancer. Mol Cancer Ther;8(3):626-635. 2009-01-01 [PMID: 19276158]

Bioinformatics

• Gene Symbol: FH
• Entrez:
  • 2271(Human)
• Uniprot:
  • AAH03108(Human)