FTSJD2 Antibody

Western Blot: FTSJD2 Antibody [NB100-79786] - Detection of Human KIAA0082 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KIAA0082 antibody NB100-79786 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry-Paraffin: FTSJD2 Antibody [NB100-79786] - Human breast carcinoma (left) and mouse CT26 colon carcinoma (right). Antibody used at a dilution of 1:1000 (1ug/ml).

Immunoprecipitation: FTSJD2 Antibody [NB100-79786] - Detection of Human KIAA0082 on HeLa whole cell lysate using NB100-79786. KIAA0082 was also IPed using NB100-79785 and NB100-79785.

• Reactivity: Hu, Mu, Pm
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

FTSJD2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 100 and 150 of human KIAA0082 using the numbering given in TrEMBL entry Q8N1G2 (GeneID 23070).
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FTSJD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 1-5 ug/mg lysate
  • Western Blot 1:5000-1:25000

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Panda and Orangutan.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FTSJD2 Antibody

• Cap1 2'O-ribose methyltransferase 1
• cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1
• EC 2.1.1.57
• FtsJ methyltransferase domain containing 2
• FtsJ methyltransferase domain-containing protein 2
• hMTr1
• Interferon-stimulated gene 95 kDa protein
• ISG95
• KIAA0082FLJ22156
• MTR1
• S-adenosyl-L-methionine-dependent methyltransferase FTSJD2

Background

KIAA0082 was first identified as a hypothetical protein predicted from the in silico analysis of long cDNAs isolated in the Kazusa cDNA sequencing project. KIAA0082 contains a G-patch and WW domain and several predicted phosphoserine sites. KIAA0082 is also known as FLJ22156.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FTSJD2 Antibody (NB100-79786)(1)

Publication using NB100-79786

• Toczydlowska-Socha D, Zielinska MM, Kurkowska M et al. Human RNA cap1 methyltransferase CMTr1 cooperates with RNA helicase DHX15 to modify RNAs with highly structured 5' termini. Philos. Trans. R. Soc. Lond., B, Biol. Sci. 2018-11-05 [PMID: 30397098] (IP, Human)

Review for FTSJD2 Antibody (NB100-79786) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Daiana Drehmer WB Human 06/07/2022

Summary

• Application: Western Blot
• Sample Tested: breast cancer MCF7 cells
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: FTSJD2
• Entrez:
  • 23070(Human)
• Uniprot:
  • Q8N1G2(Human)