FSHR Antibody (626723) [Unconjugated]

FSH R was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human FSH R Monoclonal Antibody (Catalog # MAB6559) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 626723
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

FSHR Antibody (626723) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human FSH R
  Accession # M65085
• Specificity: Detects human FSH R in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FSHR
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FSHR Antibody (626723) [Unconjugated]

• follicle stimulating hormone receptor
• follicle-stimulating hormone receptor
• Follitropin receptor
• FSH R
• FSH receptor
• FSHR
• FSH-R
• LGR1
• LGR1FSHRO
• MGC141667
• MGC141668
• ODG1

Background

Follicle stimulating hormone receptor (FSH R), also called follitropin receptor or LGR1, is a 695 amino acid (aa), ~87 kDa 7-transmembrane receptor of the leucine-rich repeat glycoprotein hormone receptor subfamily of GPCRs. It is expressed by ovarian granulosa cells and is essential for ovarian follicle maturation. In the male, it is expressed by Sertoli cells and plays a minor role in male fertility. Human FSH R shares 86% aa sequence identity with mouse and rat FSH R within the 349 aa N‑terminal extracellular domain. A 754 aa isoform contains an inserted sequence at aa 75.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FSHR Antibody (MAB6559)(3)

Publications using MAB6559

• L Man, N Lustgarten, E Kallinos, Z Redhead-La, S Liu, B Schattman, D Redmond, K Hancock, N Zaninovic, G Schattman, Z Rosenwaks, D James Comparison of Human Antral Follicles of Xenograft versus Ovarian Origin Reveals Disparate Molecular Signatures Cell Rep, 2020-08-11;32(6):108027. 2020-08-11 [PMID: 32783948] (Flow Cytometry, ICC, IHC, Human)
• Kollmann Z, Bersinger N, McKinnon B, Schneider S, Mueller M, von Wolff M Anti-Mullerian hormone and progesterone levels produced by granulosa cells are higher when derived from natural cycle IVF than from conventional gonadotropin-stimulated IVF. Reprod Biol Endocrinol, 2015-03-24;13(0):21. 2015-03-24 [PMID: 25889012] (IHC, Human)
• Hao Hong, Yongjun Yan, Sixiang Shi, Stephen A. Graves, Lazura K. Krasteva, Robert J. Nickles et al. PET of Follicle-Stimulating Hormone Receptor: Broad Applicability to Cancer Imaging Molecular Pharmaceutics 2015-02-02 [PMID: 25581441]

Bioinformatics

• Gene Symbol: FSHR
• Uniprot:
  • M65085()