FSH beta Overexpression Lysate (Native)

Images

 
Western Blot: FSH beta Overexpression Lysate (Adult Normal) [NBL1-10844] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FSH beta.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

FSH beta Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC214616 and based on accession number NM_001018080. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens follicle stimulating hormone, beta polypeptide (FSHB), transcript variant 2, mRNA.
Gene
FSHB

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
12.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for FSH beta

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FSH beta Overexpression Lysate (Native)

  • follicle stimulating hormone, beta polypeptide
  • Follicle-stimulating hormone beta subunit
  • Follitropin beta chain
  • follitropin subunit beta
  • Follitropin
  • follitropin, beta chain
  • FSH beta
  • FSHB
  • FSH-B
  • FSH-beta

Background

The pituitary glycoprotein hormone family includes follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for FSH beta Lysate (NBL1-10844) (0)

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Bioinformatics

Gene Symbol FSHB
Entrez