FSH beta Antibody (405326) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB
• Clone: 405326
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

FSH beta Antibody (405326) [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human FSH β
  Asn19-Glu129
  Accession # P01225
• Specificity: Detects human FSH beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows 50% cross-reactivity with recombinant rat (rr) FSH beta .
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FSH beta Antibody (405326) [Alexa Fluor® 405]

• follicle stimulating hormone, beta polypeptide
• Follicle-stimulating hormone beta subunit
• Follitropin beta chain
• follitropin subunit beta
• Follitropin
• follitropin, beta chain
• FSH beta
• FSHB
• FSH-B
• FSH-beta

Background

Follicle-stimulating hormone (FSH; also follitropin) is a 33 kDa heterodimer belonging to the glycoprotein hormones subunit beta family (1). The heterodimer is composed of two glycoproteins linked noncovalently: a common alpha subunit, which is also a component of luteinizing hormone, thyroid stimulating hormone, and chorionic gonadotropin; and a unique beta subunit that confers the protein’s specific biological action and is responsible for the interaction with FSH-receptor. The alpha subunit is 20 kDa and is synthesized as a 116 amino acid (aa) precursor that contains a 24 aa signal sequence and a 92 aa mature chain, which contains two potential sites of N-linked glycosylation (1-2). The beta subunit is 23 kDa and is synthesized as a 129 aa precursor with an 18 aa signal sequence and a 111 aa mature chain, which also contains two potential sites of N-linked glycosylation (1). Human FSH beta shares approximately 90% aa sequence identity with mouse and rat FSH beta. FSH is secreted by gonadotropes of the anterior pituitary gland.  Its release is controlled by pulses of gonadotropin-releasing hormone (GnRH), and those pulses, in turn, are subject to the estrogen feedback from the gonads. FSH regulates the development, growth, pubertal maturation, and reproductive processes of the human body. It promotes follicle maturation and spermatogenesis through interactions with FSH-receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.