Frizzled-9 Antibody

Western Blot: Frizzled-9 Antibody [NBP2-16533] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Frizzled 9 antibody [N1N2], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NBP2-16533] - Rat duodenum. Frizzled 9 antibody [N1N2], N-term diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NBP2-16533] - U374 xenograft, using Frizzled 9 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Product Discontinued

Frizzled-9 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Frizzled-9. The exact sequence is proprietary.
• Localization: Cell membrane; Multi-pass membrane protein
• Predicted Species: Mouse (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FZD9
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Frizzled-9 Antibody

• CD349 antigen
• CD349
• frizzled (Drosophila) homolog 9
• frizzled homolog 9 (Drosophila)
• Frizzled9
• Frizzled-9
• fz-9
• FZD3fzE6
• FZD9
• FzE6
• hFz9

Background

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Frizzled-9 Antibody (NBP2-16533)(1)

Publication using NBP2-16533

• Nalbach K, Schifferer M, Lichtenthaler S et al. Spatial Proteomics Reveals Disturbances in Trafficking and Interactions Along the Secretory Pathway Upon Loss of Neuropathy-Associated TECPR6 SSRN Electronic Journal 2022-03-16

Bioinformatics

• Gene Symbol: FZD9
• Uniprot:
  • O00144()