Human Frizzled-8 ELISA Kit (Colorimetric)

ELISA: Human Frizzled-8 ELISA Kit (Colorimetric) [NBP3-00385] - Samples were spiked with high concentrations of Human Frizzled-8 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Frizzled-8 ELISA Kit (Colorimetric) [NBP3-00385] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL

Human Frizzled-8 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human Frizzled-8 in samples. No significant cross-reactivity or interference between Human Frizzled-8 and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: 9.95%
• Intra-Assay: 10.09%
• Spike Recovery: 83 - 106%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: FZD8

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Frizzled-8 ELISA Kit (Colorimetric)

• frizzled (Drosophila) homolog 8
• frizzled 8
• frizzled family receptor 8
• frizzled homolog 8 (Drosophila)
• Frizzled8
• Frizzled-8
• FZ-8
• FZD8
• hFZ8

Background

"Frizzled-8 is a receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: FZD8