Frizzled-8 Antibody (JB40-16)

Western Blot: Frizzled-8 Antibody (JB40-16) [NBP2-75493] - Analysis of Frizzled 8 on mouse lung tissue lysates using anti-Frizzled 8 antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: Frizzled-8 Antibody (JB40-16) [NBP2-75493] - Analysis of paraffin-embedded human kidney tissue using anti-Frizzled 8 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Frizzled-8 Antibody (JB40-16) [NBP2-75493] - Analysis of paraffin-embedded human uterus tissue using anti-Frizzled 8 antibody. Counter stained with hematoxylin.

Product Discontinued

Frizzled-8 Antibody (JB40-16) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Frizzled-8 aa 561-694 / 694. (SwissProt: Q9H461 Human; SwissProt: Q61091 Mouse)
• Localization: Golgi apparatus. Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FZD8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Frizzled-8 Antibody (JB40-16)

• frizzled (Drosophila) homolog 8
• frizzled 8
• frizzled family receptor 8
• frizzled homolog 8 (Drosophila)
• Frizzled8
• Frizzled-8
• FZ-8
• FZD8
• hFZ8

Background

"Frizzled-8 is a receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FZD8