Frizzled-4 Antibody (145901) [Biotin]

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF
• Clone: 145901
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Biotin
• Concentration: LYOPH

Frizzled-4 Antibody (145901) [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-4
  Met1-Glu180
  Accession # Q61088
• Specificity: Detects human and mouse Frizzled-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse Frizzled-1, -2, -3, -6, -7, or -8 is observed. In flow cytometry and immunohistochemistry, this antibody also detected Frizzled-4 on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3, and also on human HEK293 cells.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: FZD4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.15 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Frizzled-4 Antibody (145901) [Biotin]

• CD344 antigen
• CD344
• EVR1
• exudative vitreoretinopathy 1
• FEVR
• frizzled (Drosophila) homolog 4
• frizzled homolog 4 (Drosophila)
• Frizzled4
• Frizzled-4
• Fz-4
• FZD4
• FZD4S
• FzE4
• GPCR
• hFz4
• MGC34390
• WNT receptor frizzled-4

Background

Frizzled-4 belongs to the Frizzled family of 7-pass transmembrane proteins and binds to Wnt-8. It is implicated in several developmental processes, notably in retinal angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Frizzled-4 Antibody (BAM194)(1)

Publication using BAM194

• Despeaux M, Chicanne G, Rouer E, De Toni-Costes F, Bertrand J, Mansat-De Mas V, Vergnolle N, Eaves C, Payrastre B, Girault JA, Racaud-Sultan C Focal adhesion kinase splice variants maintain primitive acute myeloid leukemia cells through altered wnt signaling. Stem Cells, 2012-08-01;30(8):1597-610. 2012-08-01 [PMID: 22714993] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: FZD4
• Uniprot:
  • Q61088()