FPRL1/FPR2 Antibody (304405) [Unconjugated]

Human peripheral blood monocytes were stained with Mouse Anti-Human FPRL1/ FPR2 Monoclonal Antibody (Catalog # MAB3479, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 304405
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

FPRL1/FPR2 Antibody (304405) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human FPRL1
  Met1-Met351
  Accession # P25090
• Specificity: Stains FPRL1 transfectants but not irrelevant transfectants. It also stains monocytes and granulocytes.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FPR2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FPRL1/FPR2 Antibody (304405) [Unconjugated]

• ALXR
• FMLP-R-I
• FMLP-R-II
• FMLPX
• formyl peptide receptor 2
• Formyl peptide receptor-like 1RFP
• FPR2
• FPR2A
• FPRH1FMLP-related receptor I
• FPRL1
• FPRL1LXA4 receptor
• HM63
• HM63FPRH2
• lipoxin A4 receptor (formyl peptide receptor related)
• Lipoxin A4 receptor
• LXA4 Receptor
• LXA4RFMLP-R-I
• N-formyl peptide receptor 2
• RFP

Background

FPRL1 is a 7TM protein expressed on mononuclear phagocytes and microglial cells. It interacts with formyl peptides to attract phagocytes to sites of infection and promote inflammatory reactions. FPRL1 also interacts with amyloid beta peptides and has been implicated in phagocyte attraction to sites of amyloid plaques in Alzheimer’s disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FPRL1/FPR2 Antibody (MAB3479)(4)

Publications using MAB3479

• LK Volpato, VV Horewicz, F Bobinski, DF Martins, AP Piovezan Annexin A1, FPR2/ALX, and inflammatory cytokine expression in peritoneal endometriosis J. Reprod. Immunol., 2018-08-03;129(0):30-35. 2018-08-03 [PMID: 30096622] (Western Blot, Human)
• HY Lee, YS Jeong, M Lee, HS Kweon, YH Huh, JS Park, JE Hwang, K Kim, YS Bae Intracellular formyl peptide receptor regulates na�ve CD4 T cell migration Biochem. Biophys. Res. Commun., 2018-02-08;0(0):. 2018-02-08 [PMID: 29427663] (Flow Cytometry, Human)
• Prevete N, Liotti F, Visciano C, Marone G, Melillo R, de Paulis A The formyl peptide receptor 1 exerts a tumor suppressor function in human gastric cancer by inhibiting angiogenesis. Oncogene, 2014-09-29;34(29):3826-38. 2014-09-29 [PMID: 25263443] (Flow Cytometry, Human)
• Ho KJ, Spite M, Owens CD, Lancero H, Kroemer AH, Pande R, Creager MA, Serhan CN, Conte MS Aspirin-triggered lipoxin and resolvin E1 modulate vascular smooth muscle phenotype and correlate with peripheral atherosclerosis. Am. J. Pathol., 2010-08-13;177(4):2116-23. 2010-08-13 [PMID: 20709806] (Western Blot, Human)

FAQs for FPRL1/FPR2 Antibody (MAB3479). (Showing 1 - 1 of 1 FAQs).

1. I am looking for an FPRL1 antibody that is reactive to mouse only. Does Novus have the antibody in question?
   • To answer your question, unfortunately all our FPRL1 antibodies are polyclonal and would detect both mouse and human formyl peptide receptor-like-1 and we do not have any mouse monoclonal antibody for it.

Bioinformatics

• Gene Symbol: FPR2
• Uniprot:
  • P25090()