FOXO4 Antibody - BSA Free

Immunohistochemistry-Paraffin: FOXO4 Antibody [NBP2-32614] - Analysis in human placenta and liver tissues using NBP2-32614 antibody. Corresponding FOXO4 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: FOXO4 Antibody [NBP2-32614] - Staining of human cerebellum shows no positivity in purkinje cells as expected.

Immunohistochemistry-Paraffin: FOXO4 Antibody [NBP2-32614] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: FOXO4 Antibody [NBP2-32614] - Staining of human testis shows moderate to strong nuclear positivity in Leydig cells.

Immunohistochemistry-Paraffin: FOXO4 Antibody [NBP2-32614] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

FOXO4 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: GNENSATEAAAIIDLDPDFEPQSRPRSCTWPLPRPEIANQPSEPPEVEPDLGEKVHTEGRSEPILLPSRLPEPAGGPQPGILGAVTGPRKGGSRRN
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FOXO4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  FOXO4 Protein (NBP2-32614PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for FOXO4 Antibody - BSA Free

• AFX
• AFX1myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 7
• Fork head domain transcription factor AFX1
• forkhead box O4
• forkhead box protein O4
• MLLT7MGC120490
• myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 7

Background

AFX aka FOXO4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOXO4