FOXO4 Antibody - BSA Free

Western Blot: FOXO4 Antibody [NBP1-77175] - A-20 cell lysate with FOXO4 antibody at (A) 0.5 and (B) 1 ug/mL.

Immunocytochemistry/ Immunofluorescence: FOXO4 Antibody - BSA Free [NBP1-77175] - Immunocytochemistry of FOXO4 in HeLa cells with FOXO4 antibody at 10 u/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

FOXO4 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human FOXO4. The immunogen is located within amino acids 50 - 100 of FOXO4. Amino Acid Squence: LPSRLPEPAGGPQPG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FOXO4
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10-20 ug/ml
  • Immunofluorescence 20 ug/ml
  • Western Blot 0.5-1 ug/ml
• Control Peptide:

  FOXO4 Peptide (NBP1-77175PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for FOXO4 Antibody - BSA Free

• AFX
• AFX1myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 7
• Fork head domain transcription factor AFX1
• forkhead box O4
• forkhead box protein O4
• MLLT7MGC120490
• myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 7

Background

FOXO4 is a ubiquitously expressed protein member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. In the absence of PI3K/AKT activation, FOXO4 localizes in the nucleus where it functions as a transcription factor. FOXO4 can also be phosphorylated by JNK following induction of reactive oxygen species (ROS), resulting in transcriptional activation and the induction of a negative feedback mechanism to counteract the ROS. It is through this mechanism that FOXO4 is thought to sensitize cancer cells to doxorubicin-mediated toxicity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOXO4
• Uniprot:
  • NP_005929(Human)