FOXO4 Antibody (S03-3B2)

Western Blot: FOXO4 Antibody (S03-3B2) [NBP3-14891] - Western blot detection of FOXO4 in K562, C6, 3T3 cell lysates using FOXO4 Rabbit mAb (1:1000 diluted). Predicted band size: 54kDa. Observed band size: 65kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S03-3B2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

FOXO4 Antibody (S03-3B2) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human FOXO4 (Uniprot#: P98177)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FOXO4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000-1/5000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for FOXO4 Antibody (S03-3B2)

• AFX
• AFX1myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 7
• Fork head domain transcription factor AFX1
• forkhead box O4
• forkhead box protein O4
• MLLT7MGC120490
• myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 7

Background

AFX aka FOXO4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOXO4