FOXO1A/3/4 Antibody - Azide and BSA Free

Western Blot: FOXO1A/3/4 Antibody [NBP3-05667] - Western blot analysis of extracts of various cell lines, using FOXO1A/3/4 antibody (NBP3-05667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Immunohistochemistry-Paraffin: FOXO1A/3/4 Antibody [NBP3-05667] - Immunohistochemistry of paraffin-embedded mouse spinal cord using FOXO1A/3/4 Rabbit pAb (NBP3-05667) at dilution of 1:100 (40x lens).

Immunohistochemistry-Paraffin: FOXO1A/3/4 Antibody [NBP3-05667] - Immunohistochemistry of paraffin-embedded rat brain using FOXO1A/3/4 Rabbit pAb (NBP3-05667) at dilution of 1:100 (40x lens).

Immunohistochemistry-Paraffin: FOXO1A/3/4 Antibody [NBP3-05667] - Immunohistochemistry of paraffin-embedded human colon carcinoma using FOXO1A/3/4 Rabbit pAb (NBP3-05667) at dilution of 1:100 (40x lens).

Immunohistochemistry: FOXO1A/3/4 Antibody - Azide and BSA Free [FOXO1A/3/4] - Immunohistochemistry analysis of paraffin-embedded human placenta using FOXO1A/3/4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry: FOXO1A/3/4 Antibody - Azide and BSA Free [FOXO1A/3/4] - Immunohistochemistry analysis of paraffin-embedded mouse liver using FOXO1A/3/4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry: FOXO1A/3/4 Antibody - Azide and BSA Free [FOXO1A/3/4] - Immunohistochemistry analysis of paraffin-embedded rat testis using FOXO1A/3/4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

FOXO1A/3/4 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit FOXO1A/3/4 Antibody - Azide and BSA Free (NBP3-05667) is a polyclonal antibody validated for use in IHC and WB. Anti-FOXO1A/3/4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide of human FOXO1A/3/4 (NP_002006.2).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FOXO1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for FOXO1A/3/4 Antibody - Azide and BSA Free

• FKH1
• FKHRforkhead box protein O1
• forkhead box O1
• Forkhead box protein O1A
• forkhead homolog in rhabdomyosarcoma
• Forkhead in rhabdomyosarcoma
• FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Publications for FOXO1A/3/4 Antibody (NBP3-05667)(1)

Publication using NBP3-05667

• Imrie H, Viswambharan H, Haywood NJ et al. Cixutumumab reveals a critical role for IGF-1 in adipose and hepatic tissue remodelling during the development of diet-induced obesity Adipocyte 2022-12-01 [PMID: 35734881] (WB, Mouse)

Bioinformatics

• Gene Symbol: FOXO1