FoxO1/FKHR Antibody (7H3)

Images

 
Immunocytochemistry/ Immunofluorescence: FOXO1A Antibody (7H3) [NB200-307] - Analysis of paraformaldehyde-fixed HeLa, using antibody (7H3) at 1:100 dilution.
Immunohistochemistry-Paraffin: FOXO1A Antibody (7H3) [NB200-307] - Analysis of paraffin-embedded Colon ca, using antibody (7H3) at 1:50 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
7H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

FoxO1/FKHR Antibody (7H3) Summary

Immunogen
Amino acids 242-655 of FKHR expressed in E. coli.
Specificity
This is specific for FKHR.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FOXO1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Application Notes
WB: Predicted MW is 70 kDa.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
FoxO1/FKHR Lysate (NBL1-10813)
Kidney Lysate (NB820-59231)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for FoxO1/FKHR Antibody (7H3)

  • FKH1
  • FKHR
  • FKHRforkhead box protein O1
  • forkhead box O1
  • Forkhead box protein O1A
  • forkhead homolog in rhabdomyosarcoma
  • Forkhead in rhabdomyosarcoma
  • FoxO1
  • FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma

Background

NB 200-307 recognizes FKHR (ForKHead in Rhabdomyosarcoma), a 70kDa protein, which is one member in a subfamily of the forkhead homeotic gene family of transcription factors. Recent experiments have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the carboxyl terminal region of the FKHR protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FoxO1/FKHR Antibody (NB200-307) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB200-307AF405
Alexa Fluor 488 Labeled NB200-307AF488
Alexa Fluor 647 Labeled NB200-307AF647
Alexa Fluor 700 Labeled NB200-307AF700
DyLight 350 Labeled NB200-307UV
DyLight 405 Labeled NB200-307V
DyLight 488 Labeled NB200-307G
DyLight 680 Labeled NB200-307FR
DyLight 755 Labeled NB200-307IR

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Bioinformatics Tool for FoxO1/FKHR Antibody (NB200-307)

Discover related pathways, diseases and genes to FoxO1/FKHR Antibody (NB200-307). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on FoxO1/FKHR.

FOXO1/FKHR (fork head in Rhabdomyosarcoma)
FOXO1 belongs to the very large Forkhead family of transcription factors which contain a conserved distinct DNA-binding domain known as the Forkhead Box, or FOX. The Forkhead domain is a 100 amino acid long motif capable of binding and bending DNA,...  Read full blog post.

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Bioinformatics

Gene Symbol FOXO1
Entrez