FoxC2 Antibody

Images

 
Western Blot: FoxC2 Antibody [NB100-1269] - HEK293 lysate (10 ug protein in RIPA buffer) over expressing Human FOXC2 with DYKDDDDK tag probed with EB06007 in Lane A,and probed with anti- DYKDDDDK Tag in lane C. ...read more
Immunohistochemistry-Paraffin: FoxC2 Antibody [NB100-1269] - staining of paraffin embedded fetal renal tissue using anti- FoxC2 antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

FoxC2 Antibody Summary

Immunogen
Peptide with sequence RHAAPYSYDCTKY corresponding to C-Terminus according to NP_005242.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FOXC2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Peptide ELISA detection limit 1:2000
Application Notes
WB: In transfected HEK293 transiently expressing full-length human FOXC2 (myc and DYKDDDDK tagged), a band of approx. 65 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. ICC/IF and IHC-F usage are reported in scientific literature (PMID: 23862012).
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
FoxC2 Lysate (NBL1-10802)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-1269 in the following applications:

Publications
Read Publications using
NB100-1269 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Bovine. Mouse reactivity reported in the scientific literature (PMID: 23862012).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FoxC2 Antibody

  • Fkh14
  • FKHL14LD
  • forkhead box C2 (MFH-1, mesenchyme forkhead 1)
  • forkhead, Drosophila, homolog-like 14
  • Forkhead-related protein FKHL14
  • FoxC2
  • LD
  • Mesenchyme fork head protein 1
  • mesenchyme forkhead 1
  • MFH-1 protein
  • MFH1
  • MFH-1
  • MFH1forkhead box protein C2
  • Transcription factor FKH-14

Background

FOXC2 belongs to the forkhead family of transcription factors which is characterized by a distinct DNA binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA

Publications for FoxC2 Antibody (NB100-1269)(4)

Reviews for FoxC2 Antibody (NB100-1269) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 2 application: IF, IHC-P.

Reviews using NB100-1269:
Filter by Applications
IF
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence FoxC2 NB100-1269
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reviewed by:
Anonymous
IF Human 07/22/2016
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Summary

ApplicationImmunofluorescence
Sample TestedHuman Embryonic Kidneys Week 17.1
SpeciesHuman
LotS3G2

Blocking

Blocking DetailsBSA 5%, 1 Hour, Room Temp

Primary Anitbody

Dilution RatioDilution: 1:50, 1:200 and 3:200, One Hour at room temp and Overnight at 4C

Secondary Antibody

Secondary DescriptionAlexa Fluor by Life Technologies
Secondary Manufacturer Cat#A11058 and A11055
Secondary Concentration1:500

Details

Detection NotesImmunofluorescence microscopy, a slide without primary antibody was used as a negative control.
Fixation DetailsFormalin 10%
Wash Description1X PBS, 3 times for 5 minutes eachAlexa Fluor by Life Technologies A11058 and A11055
Immunohistochemistry-Paraffin FoxC2 NB100-1269
Enlarge
reviewed by:
Anonymous
IHC-P Human 02/10/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedfetal renal tissue
SpeciesHuman
LotS3G2

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FoxC2 Antibody (NB100-1269) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on FoxC2.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Anonymous
07/22/2016
Application: IF
Species: Human

Anonymous
02/10/2016
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol FOXC2
Entrez
Uniprot