FOLR2 Antibody

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Western blot shows lysates of human placental tissue. PVDF membrane was probed with 1 µg/mL of Human FOLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5697) followed by HRP-conjugated Anti-Sheep IgG ...read more
FOLR2 was detected in immersion fixed human neutrophils using Human FOLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5697) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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FOLR2 Antibody Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human FOLR2
Gln22-His228
Accession # P14207
Specificity
Detects human FOLR2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FOLR1 and rhFOLR3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
FOLR2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publication using
AF5697 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FOLR2 Antibody

  • beta-HFR
  • FBP
  • FBP/PL-1
  • folate receptor 2 (fetal)
  • Folate receptor 2
  • folate receptor beta
  • folate receptor, beta
  • Folate receptor, fetal/placental
  • folate-binding protein, fetal/placental
  • FOLR2
  • FR-beta
  • FR-P3
  • Placental folate-binding protein

Background

Folate Receptor 2 (FOLR2), also known as Folate Receptor beta, is a 38 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR2 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3, 4). Human FOLR2 shares 83% amino acid sequence identity with mouse and rat FOLR2. FOLR2 is predominantly expressed in placenta, cells of the neutrophilic lineage, and some CD34+ hematopoietic progenitor cells (4‑6). It is upregulated on myeloid  leukemias, head and neck squamous cell carcinomas, and several nonepithelial cancers (3, 5, 7). It is also upregulated on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma (8‑10). FOLR2 knockout mice do not show gross morphological defects, but they exhibit increased sensitivity to arsenate-induced teratogenicity (11, 12).
  1. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
  2. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
  3. Shen, F. et al. (1994) Biochemistry 33:1209.
  4. Ratnam, M. et al. (1989) Biochemistry 28:8249.
  5. Ross, J.F. et al. (1999) Cancer 85:348.
  6. Reddy, J.A. et al. (1999) Blood 93:3940.
  7. Ross, J.F. et al. (1994) Cancer 73:2432.
  8. Nakashima-Matsushita, N. et al. (1999) Arthritis Rheum. 42:1609.
  9. van der Heijden, J.W. et al. (2009) Arthritis Rheum. 60:12.
  10. Nagai, T. et al. (2009) Cancer Immunol. Immunother. Feb 24 epub.
  11. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
  12. Wlodarczyk, B. et al. (2001) Toxicol. Appl. Pharmacol. 177:238.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FOLR2 Antibody (AF5697)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol FOLR2
Uniprot