FOLR1 Antibody (548908) [Phycoerythrin]

MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5646P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5646P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

FOLR1 knockout MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5646P, filled histogram) or isotype control antibody (IC002P, open histogram). No staining in the FOLR1 knockout MCF-7 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

HeLa cells were stained with Mouse Anti-Human FOLR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB5646P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, KO
• Clone: 548908
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

FOLR1 Antibody (548908) [Phycoerythrin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human FOLR1
  Arg25-Met233
  Accession # P15328
• Specificity: Detects human FOLR1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human FOLR2, 3 or 4 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOLR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells
  • Knockout Validated 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FOLR1 Antibody (548908) [Phycoerythrin]

• Adult folate-binding protein
• FBP
• folate binding protein
• folate receptor 1 (adult)
• Folate receptor 1
• folate receptor alpha
• Folate receptor, adult
• Folbp1
• FOLR
• FOLR1
• FR-alpha
• KB cells FBP
• MOv18
• Ovarian tumor-associated antigen MOv18

Background

Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37‑42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3‑6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1.  FOLR1 is predominantly expressed on epithelial cells and is dramatically up‑regulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the blood and breast milk (11).

1. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
2. Fowler, B. (2001) Kidney Int. Suppl. 78:S221.
3. Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.
4. Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.
5. Elwood, P.C. (1989) J. Biol. Chem. 264:14893.
6. Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.
7. Ross, J.F. et al. (1994) Cancer 73:2432.
8. Parker, N. et al. (2005) Anal. Biochem. 338:284.
9. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
10. Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.
11. Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FOLR1 Antibody (FAB5646P)(7)

Publications using FAB5646P

• Xiaodong Yang, Zhao An, Zhengyang Hu, Junjie Xi, Chenyang Dai, Yuming Zhu Expression Analysis of Ligand-Receptor Pairs Identifies Cell-to-Cell Crosstalk between Macrophages and Tumor Cells in Lung Adenocarcinoma Journal of Immunology Research 9/22/2022 [PMID: 36249427]
• B Bortot, M Mongiat, E Valencic, S Dal Monego, D Licastro, M Crosera, G Adami, E Rampazzo, G Ricci, F Romano, GM Severini, S Biffi Nanotechnology-Based Cisplatin Intracellular Delivery to Enhance Chemo-Sensitivity of Ovarian Cancer Int J Nanomedicine, 2020-07-07;15(0):4793-4810. 2020-07-07 [PMID: 32764921] (Flow Cytometry, ICC, Human)
• Zhencong Chen, Xiaodong Yang, Guoshu Bi, Jiaqi Liang, Zhengyang Hu, Mengnan Zhao et al. Ligand-receptor interaction atlas within and between tumor cells and T cells in lung adenocarcinoma International Journal of Biological Sciences 5/18/2020 [PMID: 32549766]
• Zhencong Chen, Xiaodong Yang, Guoshu Bi, Jiaqi Liang, Zhengyang Hu, Mengnan Zhao et al. Ligand-receptor interaction atlas within and between tumor cells and T cells in lung adenocarcinoma International Journal of Biological Sciences 2020-05-18 [PMID: 32549766] (Flow Cytometry, Human)
• Z Chen, M Zhao, M Li, Q Sui, Y Bian, J Liang, Z Hu, Y Zheng, T Lu, Y Huang, C Zhan, W Jiang, Q Wang, L Tan Identification of differentially expressed genes in lung adenocarcinoma cells using single-cell RNA sequencing not detected using traditional RNA sequencing and microarray Lab. Invest., 2020-04-23;0(0):. 2020-04-23 [PMID: 32327726] (Flow Cytometry, Human)
• M Ando, K Nagata, K Nihira, Y Suzuki, Y Kanda, M Adachi, T Kubota, N Kameyama, M Nakano, H Ando, K Yamano, T Ishii, R Nakai, K Nakamura Potent Therapeutic Activity Against Peritoneal Dissemination and Malignant Ascites by the Novel Anti-Folate Receptor Alpha Antibody KHK2805 Transl Oncol, 2017-07-12;10(5):707-718. 2017-07-12 [PMID: 28710915] (Flow Cytometry, Human)
• Lei Cai, Theodoros Michelakos, Cristina R. Ferrone, Liyuan Zhang, Vikram Deshpande, Qi Shen et al. Expression status of folate receptor alpha is a predictor of survival in pancreatic ductal adenocarcinoma Oncotarget 6/6/2017 [PMID: 28430580]

Bioinformatics

• Gene Symbol: FOLR1
• Uniprot:
  • P15328(Human)