FOLR1 Antibody (548908) [Phycoerythrin]

Images

 
MDA‑MB‑231 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB5646P, filled histogram) or isotype control antibody (Catalog # IC002P, open ...read more
Genetic Strategies: FOLR1 knockout MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5646P, filled histogram) or isotype control antibody ...read more
MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5646P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, KO
Clone
548908
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin
Validated by:
     

Genetic Strategies

   

FOLR1 Antibody (548908) [Phycoerythrin] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human FOLR1
Arg25-Met233
Accession # P15328
Specificity
Detects human FOLR1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human FOLR2, 3 or 4 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FOLR1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
  • Knockout Validated 10 uL/10^6 cells
Publications
Read Publications using
FAB5646P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FOLR1 Antibody (548908) [Phycoerythrin]

  • Adult folate-binding protein
  • FBP
  • folate binding protein
  • folate receptor 1 (adult)
  • Folate receptor 1
  • folate receptor alpha
  • Folate receptor, adult
  • Folbp1
  • FOLR
  • FOLR1
  • FR-alpha
  • KB cells FBP
  • MOv18
  • Ovarian tumor-associated antigen MOv18

Background

Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37‑42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3‑6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1.  FOLR1 is predominantly expressed on epithelial cells and is dramatically up‑regulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the blood and breast milk (11).
  1. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
  2. Fowler, B. (2001) Kidney Int. Suppl. 78:S221.
  3. Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.
  4. Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.
  5. Elwood, P.C. (1989) J. Biol. Chem. 264:14893.
  6. Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.
  7. Ross, J.F. et al. (1994) Cancer 73:2432.
  8. Parker, N. et al. (2005) Anal. Biochem. 338:284.
  9. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
  10. Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.
  11. Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FOLR1 Antibody (FAB5646P)(2)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB5646U
Alexa Fluor 405 FAB5646V
Alexa Fluor 488 FAB5646G
Alexa Fluor 594 FAB5646T
Alexa Fluor 647 FAB5646R
Alexa Fluor 700 FAB5646N
Alexa Fluor 750 FAB5646S
Allophycocyanin FAB5646A
Phycoerythrin FAB5646P

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Bioinformatics

Gene Symbol FOLR1
Uniprot