Follistatin-like 1/FSTL1 Antibody (229007)

• Reactivity: Hu
• Applications: WB
• Clone: 229007
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Follistatin-like 1/FSTL1 Antibody (229007) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Follistatin-like 1/FSTL1
  Met1-Ile308
  Accession # Q12841
• Specificity: Detects human Follistatin-like 1/FSTL1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse FSTL1 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: FSTL1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Follistatin-like 1/FSTL1 Antibody (229007)

• Flik
• FLJ50214
• Follistatin-like 1
• Follistatin-like protein 1
• follistatin-related protein 1
• Follistatin-related Protein
• FRP
• FRPFLJ52277
• FSL1
• FSTL1
• TSC-36

Background

Follistatin-like 1 (FSL1 or FSTL1), also known as FRP (follistatin-related protein), Flik (follistatin-like), and TSC-36 (TGF-beta 1-stimulated clone 36), is a secreted 45‑55 kDa extracellular glycoprotein belonging to the BM-40/SPARC/Osteonectin family (1-3). The human FSTL1 cDNA encodes 308 amino acids (aa), including a 20 aa signal sequence, a cysteine-rich Follistatin (EGF- and kazal-like) domain, two apparently non-functional EF-hand calcium-binding motifs, and a von Willebrand Factor C homology domain (1, 3). Mature human FSTL1 shares 94%, 95%, 98% and 99% aa identity with mouse, rat, bovine and equine FSTL1, respectively. FSTL1 was first identified as a TGF-beta-induced protein from a mouse osteoblast cell line (4). It is ubiquitously expressed in early mouse development, but is mainly mesenchymal later in development (5). In humans, FSTL1 is a common rheumatoid arthritis auto-antigen (2). It is reported to be either pro-inflammatory due to promoting inflammatory cytokine secretion, or to prevent autoimmune arthritis by inhibiting matrix metalloproteinase (MMP) and prostaglandin expression (6-9). In muscle and heart, it appears to be protective and promotes endothelial cell functions such as revascularization after ischemia, probably due to promoting expression and activation of the protein kinase AKT1 (10, 11). Cardiac and circulating FSTL1 is generally increased in conditions such as heart failure and acute coronary syndrome (11, 12). FSTL1 also appears to be a tumor suppressor, showing down-regulated expression in many human cancers (4, 14, 15). In vitro, it slows proliferation and MMP-dependent migration, and increases FAS-dependent apoptosis of tumor cell lines (14).

1. Zwijsen, A. et al. (1994) Eur. J. Biochem. 225:937.
2. Tanaka, M. et al. (1998) Int. Immunol. 10:1305.
3. Hambrook, H.O. et al. (2004) J. Biol. Chem. 279:11727.
4. Shibanuma, M. et al. (1993) Eur. J. Biochem. 217:13.
5. Adams, D. et al. (2007) Gene Expr. Patterns 7:491.
6. Clutter, S.D. et al. (2009) J. Immunol. 182:234.
7. Miyamae, T. et al. (2006) J. Immunol. 177:4758.
8. Kawabata, D. et al. (2004) Arthritis Rheum. 50:660.
9. Tanaka, M. et al. (2003) Int. Immunol. 15:71.
10. Ouchi, N. et al. (2008) J. Biol. Chem. 283:32802.
11. Oshima, Y. et al. (2008) Circulation 117:3099.
12. Lara-Pezzi, E. et al. (2008) Endocrinology 149:5822.
13. Widera, C. et al. (2009) Clin. Chem. 55:1794.
14. Chan, Q.K. et al. (2009) Carcinogenesis 30:114.
15. Mashimo, J. et al. (1997) Cancer Lett. 113:213.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Follistatin-like 1/FSTL1 Antibody (MAB1694)(6)

Publications using MAB1694

• Marijn C. Peters, Sofia Di Martino, Thomas Boelens, Jiabin Qin, Alain van Mil, Pieter A. Doevendans et al. Follistatin-like 1 promotes proliferation of matured human hypoxic iPSC-cardiomyocytes and is secreted by cardiac fibroblasts Molecular Therapy - Methods & Clinical Development 2022-06-09 [PMID: 35317048]
• Y Fang, S Zhang, X Li, F Jiang, Q Ye, W Ning Follistatin like-1 aggravates silica-induced mouse lung injury Sci Rep, 2017-03-24;7(1):399. 2017-03-24 [PMID: 28341862] (ELISA Development (Detection), Human)
• Wei K, Serpooshan V, Hurtado C, Diez-Cunado M, Zhao M, Maruyama S, Zhu W, Fajardo G, Noseda M, Nakamura K, Tian X, Liu Q, Wang A, Matsuura Y, Bushway P, Cai W, Savchenko A, Mahmoudi M, Schneider M, van den Hoff M, Butte M, Yang P, Walsh K, Zhou B, Bernstein D, Mercola M, Ruiz-Lozano P Epicardial FSTL1 reconstitution regenerates the adult mammalian heart. Nature, 2015-09-16;525(7570):479-85. 2015-09-16 [PMID: 26375005] (Western Blot, Mouse)
• Mark Gorelik, Ndate Fall, Mekibib Altaye, Michael G. Barnes, Susan D. Thompson, Alexei A. Grom et al. Follistatin-like Protein 1 and the Ferritin/Erythrocyte Sedimentation Rate Ratio Are Potential Biomarkers for Dysregulated Gene Expression and Macrophage Activation Syndrome in Systemic Juvenile Idiopathic Arthritis The Journal of Rheumatology 2013-07-01 [PMID: 23678162]
• Mark Gorelik, David C. Wilson, Yona K. Cloonan, Stanford T. Shulman, Raphael Hirsch Plasma Follistatin-Like Protein 1 is Elevated in Kawasaki Disease and May Predict Coronary Artery Aneurysm Formation The Journal of Pediatrics 2012-07-01 [PMID: 22316625] (ELISA Detection, Human)
• Yury Chaly, Anthony D. Marinov, Leif Oxburgh, Daniel S. Bushnell, Raphael Hirsch Follistatin-like Protein 1 Promotes Arthritis by Enhancing Inflammatory Cytokine/Chemokine Expression Arthritis & Rheumatism 2012-04-01 [PMID: 22006268]

Bioinformatics

• Gene Symbol: FSTL1
• Uniprot:
  • Q12841()