FNDC3A Antibody

Immunocytochemistry/Immunofluorescence: FNDC3A Antibody [NBP2-76931] - Imunofluorescence staining of paraffin- embedded zebrafish tissue using anti-FNDC3A rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody at 1/50 dilution for 10 hours at 4 and detected using Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

Immunohistochemistry: FNDC3A Antibody [NBP2-76931] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-FNDC3A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Product Discontinued

FNDC3A Antibody Summary

• Immunogen: Recombinant protein within zebrafish FNDC3A aa 97-329 / 619. (SwissProt: F1R5M9 Zebrafish)
• Localization: Golgi apparatus, Membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FNDC3A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
• Theoretical MW: 67 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FNDC3A Antibody

• bA203I16.1
• bA203I16.5
• fibronectin type III domain containing 3
• fibronectin type III domain containing 3A
• fibronectin type-III domain-containing protein 3A
• FNDC3
• HUGO
• Human gene expressed in odontoblasts
• KIAA0970FLJ31509

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FNDC3A