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FNDC3A Antibody

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Western Blot: FNDC3A Antibody [NBP1-88809] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Western Blot: FNDC3A Antibody [NBP1-88809] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). ...read more
Immunocytochemistry/ Immunofluorescence: FNDC3A Antibody [NBP1-88809] - Staining of human cell line U-251 MG shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: FNDC3A Antibody [NBP1-88809] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.
Western Blot: FNDC3A Antibody [NBP1-88809] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: FNDC3A Antibody [NBP1-88809] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: FNDC3A Antibody [NBP1-88809] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: FNDC3A Antibody [NBP1-88809] - Staining of human rectum shows strong granular cytoplasmic positivity in lymphoid cells.
Immunohistochemistry-Paraffin: FNDC3A Antibody [NBP1-88809] - Staining of human testis shows moderate granular cytoplasmic positivity in Leydig cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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FNDC3A Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GAGDMTTQYMPQYQSSQVYGDVDAHSTHGRSNFRDERSSKTYERLQKKLKDRQGTQKDKMSSPPSSPQKCPSPINEHNGLIKGQIAGGINTGSAKIKSGKGKGGTQVDTEIEEKDEETKAFEALLSNIVKPVA
Specificity
Specificity of human FNDC3A antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FNDC3A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.04 - 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
FNDC3A Protein (NBP1-88809PEP)
Publications
Read Publication using NBP1-88809.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for FNDC3A Antibody

  • bA203I16.1
  • bA203I16.5
  • fibronectin type III domain containing 3
  • fibronectin type III domain containing 3A
  • fibronectin type-III domain-containing protein 3A
  • FNDC3
  • HUGO
  • Human gene expressed in odontoblasts
  • KIAA0970FLJ31509

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody [NB200-106] - Antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
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Immunocytochemistry/Immunofluorescence: SLC6A12 Antibody [NBP1-88641] - Immunofluorescent cytostaining. Staining with rabbit anti-SLC6A12 (AlexaFluor 488, green) and goat anti-AKR1B1 (AlexaFluor594, red) in cultured healthy human myotubes at different time points. Myotubes treated with 300 u/ml IFNgamma and 30 ng/ml TNFalpha display low levels of SLC6A12 that is increased at the 48 h time point. Levels return back to constitutive low levels after 72 h, with staining levels similar to those in untreated cells. Staining for AKR1B1 shows continuously high levels between 24 and 72 h. In untreated cells, AKR1B1 expression levels are substantially lower. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (https://www.frontiersin.org/article/10.3389/fneur.2018.00846/full), licensed under a CC-BY licence.
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Western Blot: GABA Transporter 2 Antibody [NB100-1872] - Analysis of GABA 2 levels in 20 ug of young and old RPE cell lysates. [PMID: 22701690]
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Immunohistochemistry-Paraffin: GAT-1/SLC6A1 Antibody [NBP1-89802] - Immunohistochemical staining of human stomach shows no positivity in glandular cells as expected.
Western Blot: GAT-1/SLC6A1 Antibody [NBP1-89802] - Analysis in human liver tissue.
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Immunohistochemistry-Paraffin: p18INK4c/CDKN2C Antibody (18P118 (same as DCS-118)) [NB120-3216] - Human tonsil stained with p18 (NB120-3216) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells.
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Immunohistochemistry-Paraffin: SNF5 Antibody (3E10) [H00006598-M01] - Analysis of monoclonal antibody to SMARCB1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
Western Blot: SNF5 Antibody (3E10) [H00006598-M01] - SMARCB1 monoclonal antibody (M01), clone 3E10 Analysis of SMARCB1 expression in PC-12.
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Bioinformatics

Gene Symbol FNDC3A