FMRP [p Ser499] Antibody - Azide Free

Western Blot: FMRP [p Ser499] Antibody [NBP2-29509] - Western blot of rat hippocampal lysate showing specific immunolabeling of the ~71k FMRP protein phosphorylated at Ser499 (Control). The phosphospecificity of this labeling is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (400 units/100ul lysate for 30 min) before being exposed to the FMRP Ser499 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

• Reactivity: Hu, Mu, Rt, Ch, Hu, Bv, Ca, Ch, Pm, Sh, Xp, Ze
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

FMRP [p Ser499] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser499 conjugated to KLH. Accession # Q80WE1
• Modification: p Ser499
• Specificity: Specific for endogenous levels of the ~71 kDa FMRP protein phosphorylated at Ser499. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Preparation Method: Prepared from rabbit serum by affinity purification via sequentia chromatography on phospho- and dephosphopeptide affinity columns.
• Predicted Species: Human (100%), Sheep (100%), Canine (100%), Chicken (100%), Xenopus (100%), Primate (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FMR1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:250 - 1:1000
  • Western Blot 1:1000
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for FMRP [p Ser499] Antibody - Azide Free

• FMRPFRAXAPOF
• fragile X mental retardation 1 protein
• fragile X mental retardation 1
• MGC87458
• POF1
• premature ovarian failure 1
• Protein FMR-1

Background

Fragile X Mental Retardation Protein (FMRP) is an RNA-binding protein that plays an essential role in cognitive brain function. Mutations in the FMR1 gene, which codes for FMRP, can result in fragile X syndrome, autism, as well as other cognitive deficits (Brown et al.,1998, Goodlin-Jones et al., 2004). Phosphorylation of the highly conserved Ser499 has been shown to trigger hierarchical phosphorylation of nearby serines and may play a role in suppressing target mRNA translation (Ceman et al., 2003, Narayanan et al. 2008).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FMR1
• Entrez:
  • 24948(Rat)
• Uniprot:
  • Q80WE1(Rat)