FMRP Antibody (4G9)

Western Blot: FMRP Antibody (4G9) [NBP1-42567] - analysis using FMR1 mouse mAb against Jurkat (1) and K562 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: FMRP Antibody (4G9) [NBP1-42567] - Immunofluorescence analysis of NIH/3T3 cells using FMR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: FMRP Antibody (4G9) [NBP1-42567] - analysis of paraffin-embedded human brain tissues, showing cytoplasmic localization with DAB staining using FMR1 mouse mAb.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FMRP Antibody (4G9) Summary

• Immunogen: Purified recombinant fragment of human FMRP expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FMR1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for FMRP Antibody (4G9)

• FMRPFRAXAPOF
• fragile X mental retardation 1 protein
• fragile X mental retardation 1
• MGC87458
• POF1
• premature ovarian failure 1
• Protein FMR-1

Background

Fragile X syndrome is the most common inherited form of mental retardation. It is caused by loss of FMR1 gene activity due to either lack of expression or expression of a mutant form of the protein. In mammals, FMR1 is a member of a small protein family that consists of FMR1, FXR1, and FXR2. All three members bind RNA and contain sequence motifs that are commonly found in RNA-binding proteins, including two KH domains and an RGG box.The Drosophila genome contains a single gene homologous to the FXR family. dFMR1 is subjected to transcriptional and posttranscriptional regulation during development and it homomerizes, like its human counterpart. dFMR1 profile of expression recapitulates that of the human FXR protein family: it is highly enriched in muscles, in central nervous system and in gonads. In the larval brain, anti-dFMR1 also recognizes mushroom bodies, a centre that mediates learning and memory. These features make the fly an ideal system to analyze the role of the FXR family and to identify genes in the FMRP pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FMR1