Flotillin 2 Antibody - BSA Free

Western Blot: Flotillin 2 Antibody [NBP2-16507] - Flotillin 2 antibody immunoprecipitates Flotillin 2 protein in IP experiments.IP samples: HCT-116 whole cell extract. A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of Flotillin 2 protein by 4 ug Flotillin 2 antibody. 10 % SDS-PAGE. The immunoprecipitated Flotillin 2 protein was detected by Flotillin 2 antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP2-16507] - Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using antibody at 1:100 dilution.

Western Blot: Flotillin 2 Antibody [NBP2-16507] - Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE gel, diluted at 1:1000.

Western Blot: Flotillin 2 Antibody [NBP2-16507] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Bv, Xp
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Flotillin 2 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Flotillin 2. The exact sequence is proprietary.
• Localization: Cell membrane; Peripheral membrane protein; Membrane; caveola; Peripheral membrane protein
• Predicted Species: Rat (100%), Xenopus (91%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FLOT2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (91%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Flotillin 2 Antibody - BSA Free

• Epidermal surface antigen
• ESA1Membrane component chromosome 17 surface marker 1
• ESAchromosome 17, surface marker 1 (35kD protein identified bymonoclonal ECS-1)
• flotillin 2

Background

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Flotillin 2 Antibody (NBP2-16507)(1)

Publication using NBP2-16507

• Alsaqati M, Thomas RS, Kidd EJ. Proteins involved in endocytosis are upregulated by ageing in the normal human brain: implications for the development of Alzheimer's disease. J. Gerontol. A Biol. Sci. Med. Sci. 2017-06-26 [PMID: 28655199] (Human)

FAQs for Flotillin 2 Antibody (NBP2-16507). (Showing 1 - 1 of 1 FAQ).

1. Can you please tell us if the flotilln 2 antibody NBP2-16507 cross reacts with flotillin 1?
   • NBP2-16507 does NOT cross-react with flotillin 1.

Bioinformatics

• Gene Symbol: FLOT2
• Uniprot:
  • Q14254(Human)