Flotillin 2 Antibody (PSH03-41)

Immunohistochemistry: Flotillin 2 Antibody (PSH03-41) [NBP3-32343] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Flotillin 2 antibody (NBP3-32343) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32343) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: Flotillin 2 Antibody (PSH03-41) [NBP3-32343] - Immunocytochemistry analysis of HeLa cells labeling Flotillin 2 with Rabbit anti-Flotillin 2 antibody (NBP3-32343) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Flotillin 2 antibody (NBP3-32343) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: Flotillin 2 Antibody (PSH03-41) [NBP3-32343] - Flow cytometric analysis of HeLa cells labeling Flotillin 2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32343, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Western Blot: Flotillin 2 Antibody (PSH03-41) [NBP3-32343] - Western blot analysis of Flotillin 2 on different lysates with Rabbit anti-Flotillin 2 antibody (NBP3-32343) at 1/5,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: A431 cell lysate (15 µg/Lane) Lane 3: COS-1 cell lysate (15 µg/Lane) Lane 4: NIH/3T3 cell lysate (15 µg/Lane) Lane 5: PC-12 cell lysate (15 µg/Lane) Lane 6: Mouse lung tissue lysate (30 µg/Lane) Lane 7: Rat lung tissue lysate (30 µg/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: Lane 1-7 (left): 6 seconds; Lane 1-7 (right): 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: PSH03-41
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Flotillin 2 Antibody (PSH03-41) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human Flotillin 2 aa 1-428 / 428. (Uniprot: Q14254)
• Localization: Cell membrane, Membrane, caveola, Endosome, Membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FLOT2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Western Blot 1:2000-1:5000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Flotillin 2 Antibody (PSH03-41)

• Epidermal surface antigen
• ESA1Membrane component chromosome 17 surface marker 1
• ESAchromosome 17, surface marker 1 (35kD protein identified bymonoclonal ECS-1)
• flotillin 2

Background

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FLOT2