FLIP Antibody

Western Blot: FLIP Antibody - Unpurified [NB100-56306] - Analysis of I-FLICE/CASPER in A) human 293 and B) mouse NIH 3T3 cell lysate using this antibody.

Product Discontinued

FLIP Antibody Summary

• Immunogen: A peptide corresponding to amino acids 206-222 GRSKEQRLKEQLGAQQE of human I-FLICE was used to immunize rabbits.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CFLAR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 3 - 5 ug/ml
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FLIP Antibody

• CASHFADD-like antiapoptotic molecule 1
• CASP8 and FADD-like apoptosis regulator
• CASP8AP1
• Caspase homolog
• Caspase-eight-related protein
• Caspase-like apoptosis regulatory protein
• caspase-related inducer of apoptosis
• CASPER
• CasperFLAME1
• Cellular FLICE-like inhibitory protein
• CFLAR
• c-FLIPR
• c-FLIPS
• CLARPMACH-related inducer of toxicity
• FADD-like apoptotic molecule
• FLAME
• FLAME-1
• FLIP
• I-FLICE
• I-FLICEMRITc-FLIPFLIPc-FLIPL
• Inhibitor of FLICE
• usurpin beta
• Usurpin

Background

I-FLICE (inhibitor of FLICE) is a catalytically inactive form FLICE (Caspase 8) and Mch4/FLICE2 (Caspase 10). I-FLICE shares sequence homologies to FLICE and Mch4/FLICE2 and binds to these two molecules as demonstrated by co-immunoprecipitation analysis. However, unlike the effector proteases FLICE and Mch4/FLICE2, I-FLICE does not bind to the adaptor molecule FADD. I-FLICE inhibits both TNFR-1 and CD-95 induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FLIP Antibody (NB100-56306)(2)

Publications using NB100-56306

• Garimella SV, Gehlhaus K, Dine JL et al. Identification of novel molecular regulators of Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL)-induced apoptosis in breast cancer cells by RNAi screening. Breast Cancer Res. 2014-04-17 [PMID: 24745479] (WB, Human)
  
  Details:
  MB231 breast cancer cells, Fig 1E. The specificity of the antibody was validated with FLIP siRNA in MB231 cells by WB (Fig 1E).
• Sandur SK, Ichikawa H, Sethi G et al. Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone) suppresses NF-kappaB activation and NF-kappaB-regulated gene products through modulation of p65 and IkappaBalpha kinase activation, leading to potentiation of apoptosis induced by cytokine and chemotherape J Biol Chem. 2006-06-23 [PMID: 16624823]
  
  Details:
  IKKa [IMG-136A] IP (TNF-treated KBM-5 cells incubated with plumbagin), Fig. 4c WB (TNF-treated KBM-5 cells incubated with plumbagin), Fig. 4c 2. IKKb [IMG-129A] WB (TNF-treated KBM-5 cells incubated with plumbagin), Fig. 4c 3. cFLIP [IMG-116] WB (TNF-treated KBM-5 cells incubated with plumbagin), Fig. 5c.

Bioinformatics

• Gene Symbol: CFLAR
• Uniprot:
  • O15519()