FLASH Antibody

Product Discontinued

FLASH Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1650 and 1700 of human FLICE associated huge protein using the numbering given in entry NP_036247.1 (GeneID 9994).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASP8AP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FLASH Antibody

• CASP8 associated protein 2
• caspase 8 associated protein 2
• CED-4
• FLASHKIAA1315CASP8-associated protein 2
• FLICE associated huge
• FLICE-associated huge protein
• FLJ11208
• human FLASH
• RIP25FLASH homolog RIP25

Background

This protein is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. Studies of FLASH protein suggested that this protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FLASH Antibody (NB100-81646)(1)

Publication using NB100-81646

• Chen S, Evans HG, Evans DR et al. FLASH knockdown sensitizes cells to Fas-mediated apoptosis via down-regulation of the anti-apoptotic proteins, MCL-1 and Cflip short. PLoS One 2012-01-01 [PMID: 22427918]

Bioinformatics

• Gene Symbol: CASP8AP2
• Entrez:
  • 9994(Human)
• Uniprot:
  • Q9UKL3(Human)