FLASH Antibody

Images

 
Western Blot: FLASH Antibody [NB100-81646] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-81646 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) ...read more
Immunohistochemistry-Paraffin: FLASH Antibody [NB100-81646] - Human breast carcinoma. Antibody used at a dilution of 1:200.
Immunohistochemistry-Paraffin: FLASH Antibody [NB100-81646] - FFPE section of human prostate nodular hypertrophy. Affinity purified rabbit anti-FLASH used at a dilution of 1:250.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

FLASH Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1650 and 1700 of human FLICE associated huge protein using the numbering given in entry NP_036247.1 (GeneID 9994).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP8AP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Publications
Read Publication using NB100-81646.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FLASH Antibody

  • CASP8 associated protein 2
  • caspase 8 associated protein 2
  • CED-4
  • FLASHKIAA1315CASP8-associated protein 2
  • FLICE associated huge
  • FLICE-associated huge protein
  • FLJ11208
  • human FLASH
  • RIP25FLASH homolog RIP25

Background

This protein is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. Studies of FLASH protein suggested that this protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FLASH Antibody (NB100-81646)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional FLASH Products

FLASH NB100-81646

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Bioinformatics

Gene Symbol CASP8AP2
Entrez
Uniprot