FLASH Antibody


Western Blot: FLASH Antibody [NB100-81646] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-81646 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) ...read more
Immunohistochemistry-Paraffin: FLASH Antibody [NB100-81646] - Human breast carcinoma. Antibody used at a dilution of 1:200.
Immunohistochemistry-Paraffin: FLASH Antibody [NB100-81646] - FFPE section of human prostate nodular hypertrophy. Affinity purified rabbit anti-FLASH used at a dilution of 1:250.

Product Details

Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
0.2 mg/ml

Order Details

FLASH Antibody Summary

The immunogen recognized by this antibody maps to a region between residue 1650 and 1700 of human FLICE associated huge protein using the numbering given in entry NP_036247.1 (GeneID 9994).
Predicted Species
Primate (100%)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-buffered Saline and 0.1% BSA
0.09% Sodium Azide
0.2 mg/ml
Immunogen affinity purified


Application Notes
For IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended.
Read Publication using NB100-81646.

Reactivity Notes

Human. Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Rhesus Monkey, Gorilla and Crab-eating macaque.

Alternate Names for FLASH Antibody

  • CASP8 associated protein 2
  • caspase 8 associated protein 2
  • CED-4
  • FLASHKIAA1315CASP8-associated protein 2
  • FLICE associated huge
  • FLICE-associated huge protein
  • FLJ11208
  • human FLASH
  • RIP25FLASH homolog RIP25


This protein is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. Studies of FLASH protein suggested that this protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FLASH Antibody (NB100-81646)(1)

Reviews for FLASH Antibody (NB100-81646) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FLASH Antibody (NB100-81646) (0)

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Secondary Antibodies


Isotype Controls

Additional FLASH Antibody Products

FLASH NB100-81646

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Bioinformatics Tool for FLASH Antibody (NB100-81646)

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Research Areas for FLASH Antibody (NB100-81646)

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Blogs on FLASH

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Contact Information

Product PDFs


Gene Symbol CASP8AP2

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