Human FKBP52/FKBP4 ELISA Kit (Colorimetric)

ELISA: Human FKBP52/FKBP4 ELISA Kit (Colorimetric) [NBP3-31665] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Tissue homogenates, cell lysates, cell culture supernatants and other biological fluids
• Standard Curve Range: 0.156 - 10 ng/mL (example only; lot dependent)
• Sensitivity: 0.057 ng/mL (example only; lot dependent)

Human FKBP52/FKBP4 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 4.5 hours
• Standard Curve Range: 0.156 - 10 ng/mL (example only; lot dependent)
• Sensitivity: 0.057 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: FKBP4

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human FKBP52/FKBP4 ELISA Kit (Colorimetric)

• 51 kDa FK506-binding protein
• 52 kDa FKBP
• 59 kDa immunophilin
• EC 5.2.1.8
• FK506 binding protein 4, 59kDa
• FK506-binding protein 4 (59kD)
• FK506-binding protein 4
• FKBP4
• FKBP-4
• FKBP51
• FKBP52
• FKBP-52
• FKBP52T-cell FK506-binding protein, 59kD
• FKBP59
• FKBP5952 kDa FK506-binding protein
• HBI
• HSP binding immunophilin
• Hsp56
• HSP-binding immunophilin
• Immunophilin FKBP52
• p52
• p59
• peptidyl-prolyl cis-trans isomerase FKBP4
• peptidylprolyl cis-trans isomerase
• PPIase FKBP4
• PPIase
• rotamase

Background

Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR cochaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin Abinding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90 (4). Although FKBP52 and FKBP51 share approx. 75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90- binding characteristics (3, 5). Also, whereas FKBP51 typically has a role with the progesterone receptor, FKBP52 has been found to be linked to the progesterone, androgen and glucocorticoid receptors (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for FKBP52/FKBP4 ELISA Kit (NBP3-31665). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: FKBP4