FIP1L1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FIP1L1 Antibody [NBP3-38392] - Immunofluorescence analysis of A-549 cells using FIP1L1 Rabbit pAb.

Immunohistochemistry: FIP1L1 Antibody [NBP3-38392] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using FIP1L1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Western Blot: FIP1L1 Antibody [NBP3-38392] - Western blot analysis of various lysates, using FIP1L1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Immunohistochemistry: FIP1L1 Antibody [NBP3-38392] - Immunohistochemistry analysis of paraffin-embedded Rat testis using FIP1L1 Rabbit pAb at dilution of 1:100 (20x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry: FIP1L1 Antibody [NBP3-38392] - Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using FIP1L1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

FIP1L1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit FIP1L1 Antibody - BSA Free (NBP3-38392) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human FIP1L1 (NP_001128409.1).
  
  Sequence:
  MSAGEVERLVSELSGGTGGDEEEEWLYGDENEVERPEEENASANPPSGIEDETAENGVPKPKVTETEDDSDSDSDDDEDDVHVTIGDIKTGAPQYGSYGTAPVNLNIKTGGRVYGTTGTKVKGVDLDAPGSINGVPLLEVDLDSFEDKPWRKPGADLSDYFNYGFNEDTWKAYCEKQKRIRMGLEVIPVTSTTNKITAEDCTMEVTPGAEIQDGRFNLFKVQQGRTGNSEKETALPSTKAEFTSPPSLFKTGLPPSRNSTSSQSQTSTASRKANSSVGKWQDRYGRAESPDLRRLPGAID
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FIP1L1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 67 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for FIP1L1 Antibody - BSA Free

• DKFZp586K0717
• EC 1.1.1.86
• EC 4.2.1.33
• Factor interacting with PAP
• FIP1 like 1 (S. cerevisiae)
• FIP1
• FIP1-like 1 protein
• FLJ33619
• hFip1
• pre-mRNA 3'-end-processing factor FIP1
• Rearranged in hypereosinophilia
• Rhe

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FIP1L1