Filaggrin Antibody (FLG/1563) - Azide and BSA Free

Images

 
Immunohistochemistry-Paraffin: Filaggrin Antibody (FLG/1563) - Azide and BSA Free [NBP2-75774] - Formalin-fixed, paraffin-embedded human Skin stained with Filaggrin Mouse Monoclonal Antibody (FLG/1563).
Protein Array: Filaggrin Antibody (FLG/1563) - Azide and BSA Free [NBP2-75774] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Filaggrin Antibody (FLG/1563). Z- and S- Score: The ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P, PA
Clone
FLG/1563
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

Filaggrin Antibody (FLG/1563) - Azide and BSA Free Summary

Immunogen
Recombinant human Filaggrin protein fragment (around aa 998-1104) (exact sequence is proprietary)
Localization
Cytoplasmic
Specificity
Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FLG
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1 ug/ml
  • Protein Array
Application Notes
Positive Control: U-251-MG cells or Skin Theoretical Molecular Weight: 26-45 kDa (Processed); 350k Da (Profilaggrin) Immunohistochemistry-Paraffin: 0.5-1 ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Filaggrin Antibody (FLG/1563) - Azide and BSA Free

  • ATOD2
  • epidermal filaggrin
  • filaggrin
  • Profilaggrin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-75774AF350
Alexa Fluor 405 NBP2-75774AF405
Alexa Fluor 488 NBP2-75774AF488
Alexa Fluor 532 NBP2-75774AF532
Alexa Fluor 594 NBP2-75774AF594
Alexa Fluor 647 NBP2-75774AF647
Alexa Fluor 700 NBP2-75774AF700
Alexa Fluor 750 NBP2-75774AF750
Biotin NBP2-75774B
DyLight 350 NBP2-75774UV
DyLight 405 NBP2-75774V
DyLight 488 NBP2-75774G
DyLight 550 NBP2-75774R
DyLight 594 NBP2-75774DL594
DyLight 650 NBP2-75774C
DyLight 680 NBP2-75774FR
DyLight 755 NBP2-75774IR
FITC NBP2-75774F
HRP NBP2-75774H
Janelia Fluor 549 NBP2-75774JF549
Janelia Fluor 646 NBP2-75774JF646

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Bioinformatics

Gene Symbol FLG
Uniprot