Ficolin-2 Antibody

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Ficolin-2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Ficolin-2
  Leu26-Ala313
  Accession # Q15485
• Specificity: Detects human Ficolin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human Ficolin-3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FCN2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ficolin-2 Antibody

• 37 kDa elastin-binding protein
• Collagen/fibrinogen domain-containing protein 2
• EBP-37ficolin (collagen/fibrinogen domain-containing lectin) 2 (hucolin)
• FCN2
• FCNL
• FCNLficolin B
• ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin)
• Ficolin2
• Ficolin-2
• ficolin-B
• ficolin-beta
• Hucolin
• L-Ficolin
• P35
• Serum lectin p35

Background

Human Ficolin-2 (fibrinogen/collagen-like; previously called L-ficolin or ficolin-B) is a member of the ficolin family of secreted pattern recognition proteins that participate in the lectin complement activation pathway (1-4). Ficolin-2 is expressed in the liver and released into the circulation (2). The 35-40 kDa, 313 amino acid (aa) human Ficolin-2 contains a 25 aa signal sequence, an N-terminal collagen domain and a C-terminal fibrinogen-like domain that includes a calcium binding site and two potential N-glycosylation sites. The collagen domain mediates trimer formation. Larger homo-multimers are formed by disulfide links at the N-terminus, the most prominent of which is a 12 subunit oligomer (3, 5). Ficolin-2 binds microbial ligands that contain acetylated compounds (6). Notably, this includes N-acetyl glucosamine in compounds such as lipoteichoic acid in gram-positive bacteria. It also binds fungal 1,3-beta -D-glucan (4, 7, 8). Pathogen recognition by Ficolin-2 initiates an immune response that involves calcium-dependent interaction of Ficolin-2 with the MBL-associated serine protease (MASP) complex. This complex cleaves C4 to activate the complement pathway (4, 7, 8). In a secondary role, Ficolin-2 is known to bind late apoptotic and necrotic cells, probably through the recognition of exposed DNA. This also activates the complement cascade that assists in clearance of cells (9, 10). Mature human Ficolin-2 shares 70%, 72%, 76% and 78% aa identity with mouse, rat, cow and pig Ficolin-2, respectively. It shares 84% and 52% aa identity with human ficolin-1 and ficolin-3, respectively. Single nucleotide polymorphisms are common in human Ficolin-2. Some affect serum concentration, while others can increase or decrease ligand binding (11).

1. Endo, Y. et al. (2006) Adv. Exp. Med. Biol. 586:265.
2. Endo, Y. et al. (1996) Genomics 36:515.
3. Matsushita, M. et al. (1996) J. Biol. Chem. 271:2448.
4. Ma, Y. G. et al. (2004) J. Biol. Chem. 279:25307.
5. Hummelshoj, T. et al. (2007) Mol. Immunol. 44:401.
6. Krarup, A. et al. (2004) J. Biol. Chem. 279:47513.
7. Garlatti, V. et al. (2007) EMBO J. 26:623.
8. Lynch, N. J. et al. (2004) J. Immunol. 172:1198.
9. Jensen, M. L. et al. (2007) Mol. Immunol. 44:856.
10. Kuraya, M. et al. (2005) Immunobiology 209:689.
11. Hummelshoj, T. et al. (2005) Hum. Mol. Genet. 14:1651.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCN2
• Uniprot:
  • Q15485()