Ficolin-2 Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Ficolin-2 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Ficolin-2
Leu26-Ala313
Accession # Q15485
Specificity
Detects human Ficolin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human Ficolin-3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FCN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ficolin-2 Antibody

  • 37 kDa elastin-binding protein
  • Collagen/fibrinogen domain-containing protein 2
  • EBP-37ficolin (collagen/fibrinogen domain-containing lectin) 2 (hucolin)
  • FCN2
  • FCNL
  • FCNLficolin B
  • ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin)
  • Ficolin2
  • Ficolin-2
  • ficolin-B
  • ficolin-beta
  • Hucolin
  • L-Ficolin
  • P35
  • Serum lectin p35

Background

Human Ficolin-2 (fibrinogen/collagen-like; previously called L-ficolin or ficolin-B) is a member of the ficolin family of secreted pattern recognition proteins that participate in the lectin complement activation pathway (1‑4). Ficolin-2 is expressed in the liver and released into the circulation (2). The 35‑40 kDa, 313 amino acid (aa) human Ficolin-2 contains a 25 aa signal sequence, an N‑terminal collagen domain and a C‑terminal fibrinogen‑like domain that includes a calcium binding site and two potential N‑glycosylation sites. The collagen domain mediates trimer formation. Larger homo‑multimers are formed by disulfide links at the N‑terminus, the most prominent of which is a 12 subunit oligomer (3, 5). Ficolin‑2 binds microbial ligands that contain acetylated compounds (6). Notably, this includes N‑acetyl glucosamine in compounds such as lipoteichoic acid in gram-positive bacteria. It also binds fungal 1,3-beta -D-glucan (4, 7, 8). Pathogen recognition by Ficolin-2 initiates an immune response that involves calcium-dependent interaction of Ficolin-2 with the MBL-associated serine protease (MASP) complex. This complex cleaves C4 to activate the complement pathway (4, 7, 8). In a secondary role, Ficolin-2 is known to bind late apoptotic and necrotic cells, probably through the recognition of exposed DNA. This also activates the complement cascade that assists in clearance of cells (9, 10). Mature human Ficolin‑2 shares 70%, 72%, 76% and 78% aa identity with mouse, rat, cow and pig Ficolin-2, respectively. It shares 84% and 52% aa identity with human ficolin-1 and ficolin-3, respectively. Single nucleotide polymorphisms are common in human Ficolin‑2. Some affect serum concentration, while others can increase or decrease ligand binding (11).

  1. Endo, Y. et al. (2006) Adv. Exp. Med. Biol. 586:265.
  2. Endo, Y. et al. (1996) Genomics 36:515.
  3. Matsushita, M. et al. (1996) J. Biol. Chem. 271:2448.
  4. Ma, Y. G. et al. (2004) J. Biol. Chem. 279:25307.
  5. Hummelshoj, T. et al. (2007) Mol. Immunol. 44:401.
  6. Krarup, A. et al. (2004) J. Biol. Chem. 279:47513.
  7. Garlatti, V. et al. (2007) EMBO J. 26:623.
  8. Lynch, N. J. et al. (2004) J. Immunol. 172:1198.
  9. Jensen, M. L. et al. (2007) Mol. Immunol. 44:856.
  10. Kuraya, M. et al. (2005) Immunobiology 209:689.
  11. Hummelshoj, T. et al. (2005) Hum. Mol. Genet. 14:1651.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FCN2
Entrez
Uniprot