Fibrinogen gamma chain Antibody (4H9)

Western Blot: Fibrinogen gamma chain Antibody (4H9) [NBP2-37556] - Western blot analysis using FGG mAb against human FGG (AA: 210-437) recombinant protein. (Expected MW is 51.5 kDa)

Immunocytochemistry/Immunofluorescence: Fibrinogen gamma chain Antibody (4H9) [NBP2-37556] - Immunofluorescence analysis of 3T3-L1 cells using FGG mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: Fibrinogen gamma chain Antibody (4H9) [NBP2-37556] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using FGG mouse mAb with DAB staining.

Flow Cytometry: Fibrinogen gamma chain Antibody (4H9) [NBP2-37556] - Flow cytometric analysis of HepG2 cells using FGG mouse mAb (blue) and negative control (red).

Immunohistochemistry: Fibrinogen gamma chain Antibody (4H9) [NBP2-37556] - Immunohistochemical analysis of paraffin-embedded cerebellum tissues using FGG mouse mAb with DAB staining.

ELISA: Fibrinogen gamma chain Antibody (4H9) [NBP2-37556] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

Fibrinogen gamma chain Antibody (4H9) Summary

• Immunogen: Purified recombinant fragment of human FGG expressed in E. Coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGG
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Fibrinogen gamma chain Antibody (4H9)

• fibrinogen gamma chain
• fibrinogen, gamma polypeptide

Background

The protein encoded by the Fibrinogen gamma chain gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FGG