FHL2 Antibody

Western Blot: FHL2 Antibody [NB300-804] - (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FHL2 Antibody Summary

• Description: Novus Biologicals Goat FHL2 Antibody (NB300-804) is a polyclonal antibody validated for use in WB and ELISA. Anti-FHL2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence RDDILCPDCGKDI corresponding to C-Terminus according to NP_001441.4.
• Specificity: Reported variants represent identical protein (NP_001441.4; NP_963849.1; NP_963851.2; NP_001034581.1).
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FHL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 30 kDa band observed in human and mouse heart lysates (calculated MW of 32.2 kDa band according to NP_001441.4).
• Control:

  FGF basic/FGF2/bFGF Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FHL2 Antibody

• AAG11
• down-regulated in rhabdomyosarcoma LIM protein
• DRAL
• DRALAAG11
• FHL2
• FHL-2
• four and a half LIM domains 2
• four and a half LIM domains protein 2
• four and a half LIM-domain protein 2
• LIM domain protein DRAL
• Skeletal muscle LIM-protein 3
• SLIM3
• SLIM-3
• SLIM3aging-associated gene 11

Background

LIM proteins contain a highly conserved double zinc finger motif called the LIM domain. FHL2 might enforce beta-catenin transactivation activity in cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FHL2 Antibody (NB300-804)(1)

Publication using NB300-804

• Morgan MJ, Madgwick AJ. Slim defines a novel family of LIM-proteins expressed in skeletal muscle. Biochem Biophys Res Commun 1996-08-14 [PMID: 8753811]

Bioinformatics

• Gene Symbol: FHL2
• Entrez:
  • 2274(Human)
  • 14200(Mouse)
  • 63839(Rat)
• Uniprot:
  • NP_001441(Human)