FHL2 Antibody (11-134)

Western Blot: FHL2 Antibody (11-134) [NBP1-54577] - Western blot analysis of FHL2 expression in C2C12 cells using FHL2 Antibody (11-134).

Immunohistochemistry-Paraffin: FHL2 Antibody (11-134) [NBP1-54577] - Immunohistochemical detection of FHL2 on paraffin embedded section of a human prostate cancer with FHL2 Antibody (11-134).

Immunoprecipitation: FHL2 Antibody (11-134) [NBP1-54577] - Immunoprecipitation of FHL2 from C2C12 cells with normal mouse IgG (1) or NBP1-54577 (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE.

Product Discontinued

FHL2 Antibody (11-134) Summary

• Immunogen: This antibody was purified from hybridoma (clone 11-134) supernatant using protein-A Sepharose. This hybridoma was established by fusion of mouse myeloma cell SP2/0-Ag4 with Balb/c mouse splenocyte immunized with His-FHL2.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: FHL2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-5 ug/ml
  • Immunohistochemistry-Paraffin 1-5 ug/ml
  • Immunoprecipitation 3 ug/200-300 ul of cell extract
  • Western Blot 1-5 ug/ml
• Application Notes: For IHC-Paraffin, Heat treatment is necessary for paraffin embedded sections. Autoclave; 10 minutes in citrate buffer (pH 6.5) at 110 degrees Celsius.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 50% Glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for FHL2 Antibody (11-134)

• AAG11
• down-regulated in rhabdomyosarcoma LIM protein
• DRAL
• DRALAAG11
• FHL2
• FHL-2
• four and a half LIM domains 2
• four and a half LIM domains protein 2
• four and a half LIM-domain protein 2
• LIM domain protein DRAL
• Skeletal muscle LIM-protein 3
• SLIM3
• SLIM-3
• SLIM3aging-associated gene 11

Background

The monoclonal antibody F4B2-B11 reacts specifically with the LIM-only protein FHL2. Five proteins share the same structural organization and a high degree of sequence homology in this group: four and a half LIM domain protein (FHL) 1, FHL2, FHL3, FHL4, and activator of cAMP-responsive element (CRE) modulator (CREM) in testis (ACT). LIM domains are constituted by a conserved cysteine- and histidine-rich structure shaped in two repeated zinc fingers first identified in the proteins encoded by the Lin-11, Isl-1, and Mec-3 genes. The LIM domain has been shown to function as a protein-protein interaction domain, and has often been described in association with other functional protein motifs, such as homeobox and kinase domains. FHL2 seems to be a promiscuous coactivator because it modulates the activity of the androgen receptor, CRE-binding protein (CREB), and WT1, although some degree of specificity is present because it is unable to stimulate CREM- and Sp1-dependent transcription. FHL2 expression was originally described to be restricted to the heart but it is inducible in other cell types. FHL2 shows specific interaction with beta-catenin, which requires the intact structure of all four LIM domains of FHL2 and the N-terminus plus the first armadillo repeat region of beta-catenin. FHL2 is a muscle-specific repressor of LEF/TCF target genes and promotes myogenic differentiation by interacting with beta-catenin. Monoclonal antibody F4B2-B11 recognizes the N-terminal Zn-Finger motif, it does not crossreact in Western Blotting with the FHL1 and FHL3 proteins. The F4B2-B11 antibody is cross reactive with mouse and rat FHL2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FHL2
• Entrez:
  • 2274(Human)
  • 14200(Mouse)
• Uniprot:
  • Q14192()