FHL1 Antibody

Images

 
Western Blot: FHL1 Antibody [NB100-1461] - (0.01ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: FHL1 Antibody [NB100-1461] - Proteins interacting with HACE1 accumulate in Hace1-/-sTAC heart. Representative western blots show increased accumulation of some of the HACE1-interacting proteins in the ...read more

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

FHL1 Antibody Summary

Immunogen
Peptide with sequence CTVSRVSHPVSKARK corresponding to internal region according to AAC72886.1.
Specificity
This antibody is specific for the FHL-1 isoform B (SLIMMER) and is expected to recognise an epitope corresponding to aa 233-246 of human SLIMMER protein.
Predicted Species
Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FHL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1:100-1:2000
Application Notes
WB: Approx. 30 kDa band observed in human heart lysates (calculated MW of 36.3 kDa band according to AAC72886) is consistent with scientific literature (PMID: 1048092).
Control
FHL1 Overexpression Lysate
Human Heart Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publications using
NB100-1461 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FHL1 Antibody

  • bA535K18.1
  • FHL1
  • four and a half LIM domains 1
  • HLH1
  • HPLH1
  • KYOT
  • KYO-T
  • LIM protein SLIMMER
  • skeletal muscle LIM-protein1
  • SLIM1
  • SLIM1Isl-1 and Mec-3 domains 1
  • SLIMMER
  • XMPMA

Background

LIM proteins, named for 'LIN11, ISL1 and MEC3' are defined by the possession of a highly conserved double zinc finger motif called the LIM domain. FHL1 may have an involvement in muscle development or hypertrophy. Isoform 2 binds to RBP J and plays a negative regulatory role in the RBP J mediated transcription in mammalian systems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FHL1 Antibody (NB100-1461)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FHL1
Entrez
Uniprot