FHL1 Antibody

Western Blot: FHL1 Antibody [NB100-1461] - (0.01ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: FHL1 Antibody [NB100-1461] - Proteins interacting with HACE1 accumulate in Hace1-/-sTAC heart. Representative western blots show increased accumulation of some of the HACE1-interacting proteins in the Hace1-/-sTAC myocardium. n=3 mice per group, error bars represent s.e.m., P-value between KO and WT sTAC as indicated (one-way analysis of variance). GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms4430), licensed under a CC-BY license.

• Reactivity: Hu, Mu, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FHL1 Antibody Summary

• Description: Novus Biologicals Goat FHL1 Antibody (NB100-1461) is a polyclonal antibody validated for use in WB and ELISA. Anti-FHL1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CTVSRVSHPVSKARK corresponding to internal region according to AAC72886.1.
• Specificity: This antibody is specific for the FHL-1 isoform B (SLIMMER) and is expected to recognise an epitope corresponding to aa 233-246 of human SLIMMER protein.
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FHL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1:100-1:2000
• Application Notes: WB: Approx. 30 kDa band observed in human heart lysates (calculated MW of 36.3 kDa band according to AAC72886) is consistent with scientific literature (PMID: 1048092).
• Control:

  FHL1 Overexpression Lysate
  Human Heart Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FHL1 Antibody

• bA535K18.1
• FHL1
• four and a half LIM domains 1
• HLH1
• HPLH1
• KYOT
• KYO-T
• LIM protein SLIMMER
• skeletal muscle LIM-protein1
• SLIM1
• SLIM1Isl-1 and Mec-3 domains 1
• SLIMMER
• XMPMA

Background

LIM proteins, named for 'LIN11, ISL1 and MEC3' are defined by the possession of a highly conserved double zinc finger motif called the LIM domain. FHL1 may have an involvement in muscle development or hypertrophy. Isoform 2 binds to RBP J and plays a negative regulatory role in the RBP J mediated transcription in mammalian systems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FHL1 Antibody (NB100-1461)(2)

Publications using NB100-1461

• Zhang L, Chen X, Sharma P et al. HACE1-dependent protein degradation provides cardiac protection in response to haemodynamic stress. Nat Commun 2014-03-11 [PMID: 24614889] (WB, Mouse)
• Brown S, McGrath MJ, Ooms LM et al. Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1.Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in thecytoskeleton and in nuclear-cytoplasmic communication. J Biol Chem 1999-09-17 [PMID: 10480922]

Bioinformatics

• Gene Symbol: FHL1
• Entrez:
  • 2273(Human)
• Uniprot:
  • AAC72886.(Human)