FHL1 Antibody

Western Blot: FHL1 Antibody [NB100-1460] - (0.1ug/ml) staining of Mouse Skeletal Muscle (A), Rat Lung (B) and Rat Skeletal Muscle (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

• Reactivity: Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FHL1 Antibody Summary

• Description: Novus Biologicals Goat FHL1 Antibody (NB100-1460) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-FHL1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CNKRFVFHNEQVY, from the internal region of the protein sequence according to NP_001070830.1; NP_001274729.1.
• Specificity: This antibody is expected to recognize Mouse NP_001070830.1 iso 2 , NP_001274729.1 iso 3 and Rat NP_001029098.1 iso 1, NP_001258128.1 iso 3. Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FHL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.05 - 0.3 ug/mL
• Application Notes: WB: Approx 32kDa band observed in Mouse and Rat Skeletal Muscle and in Rat Lung lysates (calculated MW of 31.9kDa according to Mouse NP_001274729.1 and Rat NP_001258128.1). Primary incubation 1 hour at room temperature.
• Control:

  Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
  FHL1 Overexpression Lysate

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:18056057).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FHL1 Antibody

• bA535K18.1
• FHL1
• four and a half LIM domains 1
• HLH1
• HPLH1
• KYOT
• KYO-T
• LIM protein SLIMMER
• skeletal muscle LIM-protein1
• SLIM1
• SLIM1Isl-1 and Mec-3 domains 1
• SLIMMER
• XMPMA

Background

LIM proteins, named for LIN11, ISL1 and MEC3 are defined by the possession of a highly conserved double zinc finger motif called the LIM domain. FHL1 may have an involvement in muscle development or hypertrophy. Isoform 2 binds to RBP J and plays a negative regulatory role in the RBP J mediated transcription in mammalian systems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FHL1 Antibody (NB100-1460)(3)

Publications using NB100-1460

• Ko DS, Kang J, Heo HJ et al. Role of PCK2 in the proliferation of vascular smooth muscle cells in neointimal hyperplasia International journal of biological sciences 2022-08-08 [PMID: 35982907] (IHC-P, Mouse)
  
  Details:
  IHC-P Dilutions: 1:200; ICC/IF Dilutions: 1:400; WB Dilutions: 1:1000
• Gramolini AO, Kislinger T, Alikhani-Koopaei R et al. Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses. Mol Cell Proteomics. 2008-03-01 [PMID: 18056057] (WB, Mouse)
  
  Details:
  WB, mouse heart tissue: 1. IMG- 5346A (Nogo A/B): Fig. C. 2. IMG-3374 (FHL1): Figs. 3C; 4A, B; 6; 8A, B.
• Brown S, McGrath MJ, Ooms LM et al. Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1.Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in thecytoskeleton and in nuclear-cytoplasmic communication. J Biol Chem 1999-09-17 [PMID: 10480922]

Bioinformatics

• Gene Symbol: FHL1
• Entrez:
  • 2273(Human)
  • 14199(Mouse)
  • 25177(Rat)
• Uniprot:
  • NP_001440.(Human)