FHL1 Antibody

Images

 
Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human FHL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5938) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

FHL1 Antibody Summary

Immunogen
E. coli-derived recombinant human FHL1
Thr93-Asn166
Accession # Q13642
Specificity
Detects human FHL1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FHL2 and rhFHL3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FHL1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Control
FHL1 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FHL1 Antibody

  • bA535K18.1
  • FHL1
  • four and a half LIM domains 1
  • HLH1
  • HPLH1
  • KYOT
  • KYO-T
  • LIM protein SLIMMER
  • skeletal muscle LIM-protein1
  • SLIM1
  • SLIM1Isl-1 and Mec-3 domains 1
  • SLIMMER
  • XMPMA

Background

FHL1 (Four-and-a-half LIM domains protein 1; also SLIM1 and KyoT1) is a 36 kDa member of the four-and-a half class of the LIM domain-only protein family. It is highly expressed in myocardium and skeletal muscle, and is now known to bind NFATc1, promoting muscle hypertrophy. It is also noted to be a RIP140 binding partner that inhibits estrogen receptor signaling. Human FHL1 is 323 amino acids (aa) in length. It contains one C4-type Zn-finger region (aa 7-31), three distinct LIM domains (aa 40-77; 101-158; 162-212) and a bipartite NLS (aa 231-246). There is one 32 kDa splice variant that shows a 50 aa substitution for aa 231-323. This creates a fourth LIM domain. There are also alternative start sites 29 aa and 16 aa upstream of the standard site, a deletion of aa 168-296, and an 11 aa substitution for aa 81-91. Over aa 93-166, human FHL1 is 97% aa identical to mouse FHL1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF4779
Species: Hu
Applications: WB, IHC
MAB4247
Species: Hu
Applications: WB, IP
NBP1-84843
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89549
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-31336
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-78159
Species: Hu, Rt, Po, Bv
Applications: WB, EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, PLA, RIA
NBP1-80833
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-33427
Species: Hu
Applications: WB, ICC/IF, ChIP
H00009260-M01
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, IP, RNAi, S-ELISA
NB200-540
Species: Mu
Applications: Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP1-84978
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-91899
Species: Hu
Applications: WB, ICC/IF
NB100-41385
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA, KD
NBP1-88262
Species: Hu
Applications: WB, IHC, IHC-P
NB600-930
Species: Hu, Rt
Applications: WB, ELISA
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-292
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-49938
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP

Publications for FHL1 Antibody (AF5938) (0)

There are no publications for FHL1 Antibody (AF5938).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for FHL1 Antibody (AF5938) (0)

There are no reviews for FHL1 Antibody (AF5938). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FHL1 Antibody (AF5938) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional FHL1 Products

Bioinformatics Tool for FHL1 Antibody (AF5938)

Discover related pathways, diseases and genes to FHL1 Antibody (AF5938). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FHL1 Antibody (AF5938)

Discover more about diseases related to FHL1 Antibody (AF5938).
 

Pathways for FHL1 Antibody (AF5938)

View related products by pathway.

PTMs for FHL1 Antibody (AF5938)

Learn more about PTMs related to FHL1 Antibody (AF5938).

Blogs on FHL1

There are no specific blogs for FHL1, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our FHL1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol FHL1
Uniprot