Fgr Antibody (1B12) - Azide and BSA Free

Western Blot: Fgr Antibody (1B12) [H00002268-M03] - Analysis of FGR expression in transfected 293T cell line by FGR monoclonal antibody (M03), clone 1B12. Lane 1: FGR transfected lysatE (59.5 KDa). Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: Fgr Antibody (1B12) [H00002268-M03] - Analysis of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml

Western Blot: Fgr Antibody (1B12) [H00002268-M03] - Analysis of FGR expression in HeLa (Cat # L013V1).

Western Blot: Fgr Antibody (1B12) [H00002268-M03] - Analysis of FGR expression in PC-12 (Cat # L012V1).

Western Blot: Fgr Antibody (1B12) [H00002268-M03] - Analysis of FGR expression in Raw 264.7 (Cat # L024V1).

ELISA: Fgr Antibody (1B12) [H00002268-M03] - Detection limit for recombinant GST tagged FGR is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clone: 1B12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Fgr Antibody (1B12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: FGR (AAH64382, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGCVFCKKLEPVATAKEDAGLEGDFRSYGAADHYGPDPTKARPASSFAHIPNYSNFSSQAINPGFLDSGTIRGVSGIGVTLFIALYDYEA
• Specificity: FGR - Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGR
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Fgr Antibody (1B12) - Azide and BSA Free

• c-fgr
• c-src-2 proto-oncogene
• c-src2
• EC 2.7.10
• EC 2.7.10.2
• Fgr
• FLJ43153
• Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog
• MGC75096
• p55-c-fgr protein
• p55c-fgr
• p55-Fgr
• p58c-fgr
• Proto-oncogene c-Fgr
• proto-oncogene tyrosine-protein kinase FGR
• Src2
• SRC2c-fgr protooncogene
• tyrosine-protein kinase Fgr

Background

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fgr Antibody (H00002268-M03)(1)

Publication using H00002268-M03

• Jia R, Pan Q, Ding S et al. The N-terminal region of IFITM3 modulates its antiviral activity through regulating IFITM3 cellular localization. J Virol 2012-10-10 [PMID: 23055554] (WB, Human)

Bioinformatics

• Gene Symbol: FGR
• Entrez:
  • 2268(Human)
• OMIM:
  • 164940(Human)
• Uniprot:
  • AAH64382(Human)